Overview

This detailed volume explores a wide variety of techniques involving optical tweezers, a technology that has become increasingly more accessible to a broad range of researchers. Beginning with recent technical advances, the book continues by covering the application of optical tweezers to study DNA-protein interactions and DNA motors, protocols to perform protein (un)folding experiments, the application of optical tweezers to study actin- and microtubule-associated motor proteins, and well as protocols for investigating the function and mechanical properties of microtubules and intermediate filaments, and more. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Optical Tweezers: Methodsand Protocols, Second Edition serves as an ideal resource for expanding the accessibility and use of optical traps by scientists of diverse disciplines.

Full Product Details

Author:   Arne Gennerich
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   2nd ed. 2022
Volume:   2478
Weight:   1.630kg
ISBN:  

9781071622285

ISBN 10:   1071622285
Pages:   757
Publication Date:   06 September 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I: Historic Views on the Invention of Lasers and Optical Tweezers   1. The Invention of the Laser             Nick Taylor   2. Art Ashkin and the Origins of Optical Trapping and Particle Manipulation             Matthias D. Koch and Joshua W. Shaevitz   Part II: Technical Advances   3. Synthesis of Germanium Nanospheres as High Precision Optical Tweezers Probes             Swathi Sudhakar, Viktoria Wedler, Pasupathi Rajendran, and Erik Schäffer   4. Angular Optical Trapping to Directly Measure DNA Torsional Mechanics             Xiang Gao, James T. Inman, and Michelle D. Wang   5. Implementation of 3D Multi-Color Fluorescence Microscopy in a Quadruple Trap Optical Tweezers System             Anna E.C. Meijering, Julia A.M. Bakx, Tianlong Man, Iddo Heller, Gijs J.L. Wuite, and Erwin J.G. Peterman   6. One-Dimensional STED Microscopy in Optical Tweezers             Tianlong Man, Joost J. Geldhof, Erwin J.G. Peterman, Gijs J.L. Wuite, and Iddo Heller   7. Temperature Quantification and Temperature Control in Optical Tweezers             Joost J. Geldhof, Agata M. Malinowska, Gijs J.L. Wuite, Erwin J.G. Peterman, and Iddo Heller   8. High-Resolution Optical Tweezers Combined with Multi-Color Single-Molecule Microscopy             Rajeev Yadav, Kasun B. Senanayake, and Matthew J. Comstock   Part III: DNA, DNA Motors and DNA-Protein Interactions   9. Generating Negatively Supercoiled DNA Using Dual-Trap Optical Tweezers             Graeme A. King, Dian Spakman, Erwin J.G. Peterman, and Gijs J.L Wuite   10. Force-Activated DNA Substrates for In Situ Generation of ssDNA and Designed ssDNA/dsDNA Structures in an Optical-Trapping Assay             Arnulf M.K. Taylor, Stephen R. Okoniewski, Lyle Uyetake, and Thomas T. Perkins   11. Probing the Interaction between Chromatin and Chromatin-Associated Complexes with Optical Tweezers             Rachel Leicher and Shixin Liu   12. Simultaneous Mechanical and Fluorescence Detection of Helicase-Catalyzed DNA Unwinding             Lulu Bi, Zhenheng Qin, Xi-Miao Hou, Mauro Modesti, and Bo Sun   13. CRISPR/Cas9 On- and Off-Target Activity Using Correlative Force and Fluorescence Single-Molecule Microscopy             Matthew D. Newton, Benjamin J. Taylor, Maria Emanuela Cuomo, and David S. Rueda   Part IV: Protein (Un)Folding   14. Co-Temporal Single-Molecule Force and Fluorescence Measurements to Determine the Mechanism of Ribosome Translocation             Varsha P. Desai, Filipp Frank, and Carlos J. Bustamante   15. Using Single-Molecule Optical Tweezers to Study the Conformational Cycle of the Hsp90 Molecular Chaperone             Katarzyna Tych and Matthias Rief   16. Tethering Complex Proteins and Protein Complexes for Optical Tweezers Experiments             Kevin Maciuba and Christian M. Kaiser   17. Single-Molecule Manipulation Study of Chaperoned SNARE Folding and Assembly with Optical Tweezers             Huaizhou Jin, Jinghua Ge, Jie Yang, and Yongli Zhang   18. Using Optical Tweezers to Monitor Allosteric Signals through Changes in Folding Energy Landscapes             Lihui Bai, Mira Malmosi, Lydia Good, and Rodrigo A. Maillard   Part V: Cytoskeletal Motors and Proteins   19. High-Speed Optical Traps Address Dynamics of Processive and Non-Processive Molecular Motors             Lucia Gardini, Michael S. Woody, Anatolii V. Kashchuk, Yale E. Goldman, E. Michael Ostap, and Marco Capitanio   20. Microtubule Dumbbells to Assess the Effect of Force Geometry on Single Kinesin Motors             Serapion Pyrpassopoulos, Henry Shuman, and E. Michael Ostap   21. Single-Molecule Studies on the Motion and Force Generation of the Kinesin-3 Motor KIF1A             Lu Rao and Arne Gennerich   22. Ultra-Fast Force-Clamp Spectroscopy of Microtubule-Binding Proteins             Suvranta K. Tripathy, Vladimir M. Demidov, Ivan V. Gonchar, Shaowen Wu, Fazly I. Ataullakhanov, and Ekaterina L. Grishchuk   Part VI: Function and Mechanical Properties of Filaments   23. Catching the Conformational Wave: Measuring the Working Strokes of Protofilaments as They Curl Outward from Disassembling Microtubule Tips             Lucas E. Murray, Haein Kim, Luke M. Rice, and Charles L. Asbury   24. Mechanics of Single Vimentin Intermediate Filaments under Load             Anna V. Schepers, Julia Kraxner, Charlotta Lorenz, and Sarah Köster   25. Quantifying the Interaction Strength between Biopolymers             Charlotta Lorenz, Anna V. Schepers, and Sarah Köster   Part VII: Mechanosensing of Membrane Channels   26. Measuring αβ T Cell Receptor-Mediated Mechanosensing Using Optical Tweezers Combined with Fluorescence Imaging             Hannah M. Stephens, Kristine N. Brazin, Robert J. Mallis, Yinnian Feng, Debasis Banik, Ellis L. Reinherz, and Matthew J. Lang

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