Overview

This book provides a collection of comprehensive, up-to-date, and broadly applicable guides to the research and development fields of oligonucleotide (ON) therapeutics. Covering topics from the study of antisense and anti-gene effects to oligonucleotides in the context of drug discovery and development, the volume explores a wide-ranging and useful spectrum of methods and protocols needed to take full advantage of therapeutic applications involving ONs. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Oligonucleotide-Based Therapies: Methods and Protocols aims to be a great aid in the laboratory as well as an ideal reference guide when designing antisense and anti-gene oligonucleotides for therapeutic applications.

Full Product Details

Author:   Olof Gissberg ,  Rula Zain ,  Karin E. Lundin
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. 2019
Volume:   2036
Weight:   0.677kg
ISBN:  

9781493996728

ISBN 10:   149399672
Pages:   344
Publication Date:   15 August 2020
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I: Reviews   1. Chemical Development of Therapeutic Oligonucleotides             Karin E. Lundin, Olof Gissberg, C.I. Edvard Smith, and Rula Zain   2. Tissue-Specific Delivery of Oligonucleotides             Xin Xia, Nicolette Pollock, Jiehua Zhou, and John Rossi   Part II: Protocols and Strategies   3. Designing siRNA and Evaluating Its Effect on RNA Targets Using qPCR and Western Blot             Linda Vidarsdottir, Oksana Goroshchuk, Iryna Kolosenko, and Caroline Palm-Apergi   4. Antisense Oligonucleotides for Splice Modulation: Assessing Splice Switching Efficacy             Cristina S.J. Rocha   5. Assessing Oligonucleotide Binding to Double-Strand DNA             Negin Mozafari and Tea Umek   6. Modelling and Simulation of Oligonucleotide Hybrids: Outlining a Strategy             Lennart Nilsson and Alessandra Villa   7. Characterization of Peptide/Oligonucleotide Complexes Using Electron Microscopy, Dynamic Light Scattering, and Protease Resistance Assay             Kärt Padari, Ly Porosk, Piret Arukuusk, and Margus Pooga   8. Click-Shielded and Targeted Lipopolyplexes             Philipp Michael Klein and Ernst Wagner   9. Attachment of Peptides to Oligonucleotides on Solid Support Using Copper(I)-Catalyzed Huisgen 1,3-Dipolar Cycloaddition             Malgorzata Honcharenko, Dmytro Honcharenko, and Roger Stromberg   10. Characterizing Oligonucleotide Uptake in Cultured Cells: A Case Study Using AS1411 Aptamer             Elsa M. Reyes-Reyes and Paula J. Bates   11. Measuring the Action of Oligonucleotide Therapeutics in the Lung at the Cell Type-Specific Level by Tissue Disruption and Cell Sorting (TDCS)             Helen Graves, Steven Evans, Michael Fauler, Manfred Frick, and Sterghios A. Moschos   12. Delivery of Antisense Oligonucleotides Mediated by a Hydrogel System: In Vitro and In Vivo Application in the Context of Spinal Cord Injury             Pedro M.D. Moreno, Teresa Rodrigues, Marília Torrado, Isabel F. Amaral, and Ana P. Pêgo   13. Evaluation of Cell-Penetrating Peptide Delivery of Antisense Oligonucleotides for Therapeutic Efficacy in Spinal Muscular Atrophy             Suzan M. Hammond, Frank Abendroth, Michael J. Gait, and Matthew J.A. Wood   14. Injection and Infusion of Compounds to the Central Nervous System             Margherita Zamboni   15. In Vitro Assessment of the Hepatotoxicity Potential of Therapeutic Oligonucleotides             Sabine Sewing, Tanja Minz, and Franziska Boess   16. Identifying Suitable Target Regions and Analyzing Off-Target Effects             Lykke Pedersen, Peter H. Hagedorn, and Troels Koch   17. RACE-SEQ and Population-Wide Polymorphism Susceptibility Testing for Endonucleolytically Active, RNA-Targeting Therapeutics             Louise Usher, Pantazis I. Theotokis, and Sterghios A. Moschos   18. Studying the Biotransformation of Phosphorothioate-Containing Oligonucleotide Drugs by LC-MS             Christophe Husser, Erich Koller, Andreas Brink, and Simone Schadt   19. ADME: Assessing Pharmacokinetic-Pharmacodynamic Parameters of Oligonucleotides             Rasmus Jansson-Löfmark*, Christine Ahlström, and Peter Gennemark

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