Overview

This book volume provides in depth coverage of the nucleic acid field and aims to represent a broad diversity of the methodologies and a wide range of nucleic-acid-related topics within the fields of molecular biology and biomedicine. Chapters are divided in three sections detailing in vitro detection, nanotechnology and imaging, biomedical applications, and big data. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and cutting-edge, Nucleic Acid Detection and Structural Investigations: Methods and Protocols aims present aims to present methodologies combine cutting-edge innovation with sound theory and practical applications in life sciences.

Full Product Details

Author:   Kira Astakhova ,  Syeda Atia Bukhari
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2020
Volume:   2063
Weight:   0.765kg
ISBN:  

9781071601372

ISBN 10:   1071601377
Pages:   270
Publication Date:   31 October 2019
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Optomagnetic Detection of Rolling Circle Amplification Products.- Subtyping of Swine Influenza Using a High-Throughput Real-Time PCR Platform and a Single Microfluidics Device.- RT-qPCR Detection of Low Copy HIV RNA with Yin-Yang Probes.- Solid-phase Hybridization Assay for Detection of Mutated Cancer DNA by Fluorescence.- 5'-Mono- and 5'-bis-pyrene 2'-O-methyl RNA Probes for Detection of RNA Mismatches.- Combined Assay for Detecting Autoantibodies to Nucleic Acids and Apolipoprotein H in Patients with Systemic Lupus Erythematosus.- Electrochemical Detection of dsDNA-specific Antibodies.- Sequence-defined DNA-amphiphiles for Drug Delivery: Synthesis and Self-assembly.- DNA-mediated Liposome Fusion Observed by Fluorescence Spectrometry.- Advanced Fluorescence Imaging to Distinguish Between Intracellular Fractions of Antisense Oligonucleotides.- Methodology for Subcellular Fractionation and microRNA Examination of Mitochondria, Mitochondria Associated ER Membrane (MAM), ER, and Cytosol from Human Brain.- Skeletal Muscle Injury by Electroporation – A Model to Study Degeneration/Regeneration Pathways in Muscle.- Isolation and Characterization of Muscle Derived Stem Cells from Dystrophic Mouse Models.- Universal Library Preparation Protocol for Efficient High-Throughput Sequencing Of Double-stranded RNA Viruses.- Quantitation of Molecular Pathway Activation using RNA Sequencing Data.- Molecular Pathway Analysis of Mutation Data for Biomarkers Discovery and Scoring of Target Cancer Drugs.- Oncobox Method for Scoring Efficiencies of Anticancer Drugs Based on Gene Expression Data.- Global Characterization of Circulating Nucleic Acids.  

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