Overview

This detailed volume explores the large research area of Notch signaling studies in a variety of model organisms. The collected research methods and protocols include the dissection of Notch functional sites, Notch regulators, Notch activity reporters and analysis, roles of Notch in development and diseases, wet and dry lab tools and studies, and bioinformatics analysis. This book particularly emphasizes on the exploration of Notch roles in development and diseases, while acknowledging the importance of combining classic and novel molecular tools and methods, bioinformatics workflows and applications, and different model organisms to fully understand the subject area. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and cutting-edge, Notch Signaling Research: Methods and Protocols is an ideal guide for researchers in the field, with techniques that could potentially be applied to study the wider world of other signaling pathways as well.

Full Product Details

Author:   Dongyu Jia
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2022
Volume:   2472
Weight:   0.787kg
ISBN:  

9781071622001

ISBN 10:   1071622005
Pages:   298
Publication Date:   09 June 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Using the CRISPR/Cas9 System for Dissection of Functional Sites of the Notch Gene in Drosophila melanogaster             Oleg V. Andreyenkov, Elena I. Volkova, Natalya G. Andreyenkova, and Sergey A. Demakov   2. Screening Mutants by Single Fly Genomic PCR             Usha Nagarajan and Marios Georgiou   3. Generation of Properly Folded Epidermal Growth Factor-Like (EGF) Repeats and Glycosyltransferases Enables In Vitro O-Glycosylation             Chenyu Ma, Yohei Tsukamoto, and Hideyuki Takeuchi   4. Use of FLP/FRT System to Screen for Notch Signaling Regulators in the Drosophila Wing             Dongqing Mo, Jie Shen, and Junzheng Zhang   5. High-Throughput Analysis to Identify Activators of Notch Signaling             Rachael Guenter, Jacob Eide, Herbert Chen, J. Bart Rose, and Renata Jaskula-Sztul   6. Artificial Notch Signaling Activation Method Using Immobilized Ligand Beads             Takamasa Mizoguchi, Hikaru Handa, Shuhei Omaru, and Motoyuki Itoh   7. Mammalian NOTCH Receptors Activation and Signaling Protocols             María Luisa Nueda and Victoriano Baladrón   8. Somatic Clonal Analyses Using FLP/FRT and MARCM System to Understand Notch Signaling Mechanism and Its Regulation             Vartika Sharma, Nalani Sachan, Mousumi Mutsuddi, and Ashim Mukherjee   9. Analyzing the Interaction of RBPJ with Mitotic Chromatin and Its Impact on Transcription Reactivation upon Mitotic Exit             Kostiantyn Dreval, Robert J. Lake, and Hua-Ying Fan   10. Assessing the Roles of Potential Notch Signaling Components in Instructive and Permissive Pathways with Two Drosophila Pericardial Reporters             Manoj Panta, Andrew J. Kump, Kristopher R. Schwab, and Shaad M. Ahmad   11. Image-Based Single-Molecule Analysis of Notch-Dependent Transcription in Its Natural Context             ChangHwan Lee, Tina Lynch, Sarah L. Crittenden, and Judith Kimble   12. An Automatic Stage Identification MATLAB Tool to Reveal Notch Expression Pattern in Drosophila Egg Chambers             Lily Paculis, Qiuping Xu, Qian Xie, and Dongyu Jia   13. Employing the CRISPR Technology for Studying Notch Signaling in the Male Gonad of Drosophila melanogaster             Cordula Schulz   14. Immuno-Localization of Notch Signaling in Mouse Preimplantation Embryos             Elisabete Silva, Patrícia Diniz, Alexandre Trindade, Mariana Batista, Ana Torres, António Duarte, and Luís Lopes-da-Costa   15. Studying the NOTCH Signaling Pathway Activation in Kidney Biopsies             Laura Márquez-Expósito, Carolina Lavoz, Elena Cantero-Navarro, Raúl R. Rodrigues-Diez, Sergio Mezzano, and Marta Ruiz-Ortega   16. Exosomes as Carriers for Notch Molecules             Guya Diletta Marconi, Francesca Diomede, Oriana Trubiani, Cristina Porcheri, and Thimios A. Mitsiadis   17. In Vivo and Ex Vivo Experimental Approach for Studying Functional Role of Notch in Pulmonary Vascular Disease             Pritesh P. Jain, Susumu Hosokawa, Aleksandra Babicheva, Tengteng Zhao, Jiyuan Chen, Patricia A. Thistlethwaite, Ayako Makino, and Jason X.-J. Yuan   18. Metastasis Model to Test the Role of Notch Signaling in Prostate Cancer             Shiqin Liu, En-chi Hsu, Michelle Shen, Merve Aslan, and Tanya Stoyanova   19. Functional Studies of Genetic Variants Associated with Human Diseases in Notch Signaling-Related Genes Using Drosophila             Sheng-An Yang, Jose L. Salazar, David Li-Kroeger, and Shinya Yamamoto   20. Bioinformatics Tools to Understand Notch             Ashley Avila, Roxana Gonzalez Tascon, and Dongyu Jia

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