Non-Natural Nucleic Acids: Methods and Protocols

Author:   Nathaniel Shank
Publisher:   Humana Press Inc.
Edition:   2019 ed.
Volume:   1973
ISBN:  

9781493992157


Pages:   315
Publication Date:   24 April 2019
Format:   Hardback
Availability:   Manufactured on demand   Availability explained
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Non-Natural Nucleic Acids: Methods and Protocols


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Author:   Nathaniel Shank
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2019 ed.
Volume:   1973
Weight:   0.843kg
ISBN:  

9781493992157


ISBN 10:   1493992155
Pages:   315
Publication Date:   24 April 2019
Audience:   College/higher education ,  Professional and scholarly ,  Postgraduate, Research & Scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Synthesis and Enzymatic Characterization of Sugar-Modified Nucleoside Triphosphate Analogs.- Synthesis of Site-Specific Crown Ether Adducts to DNA Abasic Sites, 8-Oxo-7,8-Dihydro-2’-Deoxyguanosine and 2’-Deoxycytidine.- Synthesis of a Fluorescent Cytidine TNA Triphosphate Analogue.- Synthesis of Base-Modified dNTPs through Cross-Coupling Reactions and Their Polymerase Incorporation to DNA.- 2ʹ-C,4ʹ-C-Ethyleneoxy-Bridged 2ʹ-Deoxyribonucleic Acids (EoDNAs) with Thymine Nucleobases: Synthesis, Duplex-Forming Ability, and Enzymatic Stability.- Synthesis Protocols for Simple Uncharged Glycol Carbamate Nucleic Acids.- Synthesis of Nucleobase-Functionalized Morpholino Monomers.- Synthesis and Application of LKγT Peptide Nucleic Acids.- Aminoglycoside Functionalization as a Tool for Targeting Nucleic Acids.- Preparation and Purification of Oligodeoxynucleotide Duplexes Containing a Site-Specific, Reduced, Chemically-Stable Covalent Interstrand Cross-Link between a Guanine Residue and an Abasic Site.- Copper-Catalyzed Alkyne-Azide Cycloaddition on the Solid Phase for the Preparation of Fully Click-Modified Nucleic Acids.- Labeling Peptide Nucleic Acids with Indium-111.- Site-Specific Labeling of DNA via PCR with an Expanded Genetic Alphabet.- Flexible Nucleic Acids (FNAs) As Informational Molecules:  Enzymatic Polymerization of fNTPs on DNA Templates and Nonenzymatic Oligomerization of RNA on FNA Templates.- Artificial Nucleosides as Diagnostic Probes to Measure Translesion DNA Synthesis.- FRET Assay for Ligands Targeting the Bacterial A-Site RNA.- The Use of Serinol Nucleic Acids as Ultrasensitive Molecular Beacons.- Oligonucleotide Primers with G8AE-Clamp Modifications for RT-qPCR Detection of the Low-Copy dsRNA.- Determining Steady-State Kinetics of DNA Polymerase Nucleotide Incorporation.

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