Neutrophil: Methods and Protocols

Author:   Mark T. Quinn ,  Frank R. DeLeo
Publisher:   Springer-Verlag New York Inc.
Edition:   Third Edition 2020
Volume:   2087
ISBN:  

9781071601563


Pages:   470
Publication Date:   14 November 2020
Format:   Paperback
Availability:   Manufactured on demand   Availability explained
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Neutrophil: Methods and Protocols


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Author:   Mark T. Quinn ,  Frank R. DeLeo
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   Third Edition 2020
Volume:   2087
Weight:   0.920kg
ISBN:  

9781071601563


ISBN 10:   1071601563
Pages:   470
Publication Date:   14 November 2020
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Dedication... Preface... Table of Contents... Contributing Authors... Part I: Neutrophils and Neutrophil Disorders: Overviews 1. The Role of Neutrophils in the Immune System: An Overview Harry L. Malech, Frank R. DeLeo, and Mark T. Quinn 2. Neutrophil Defects and Diagnosis Disorders of Neutrophil Function: An Overview Mary C. Dinauer Part II: Neutrophil Isolation 3. Isolation of Human Neutrophils from Venous Blood Silvie Kremserova and William M. Nauseef 4. Isolation of Neutrophils from Non-Human Species Daniel W. Siemsen, Lilya N. Kirpotina, Natalia Malachowa, Igor A. Schepetkin, Adeline R. Porter, Benfang Lei, Frank R. DeLeo, and Mark T. Quinn 5. Isolation of Neutrophils from Larval Zebrafish and their Transplantation into Recipient Larvae for Functional Studies Hannah Darroch, Jonathan W. Astin, and Christopher J. Hall Part III: Neutrophil Chemotaxis, Phagocytosis, and Bactericidal Activity 6. Analysis of Neutrophil Transmigration through Epithelial Cell Monolayers Liliya N. Kirpotina, Douglas J. Kominsky, Mark T. Quinn, and Steve D. Swain 7. Quantification of Chemotaxis or Respiratory Burst using Ex Vivo Culture-Derived Murine Neutrophils Klaudia Szymczak, Margery G.H. Pelletier, and Peter Gaines 8. Ex Vivo Human Neutrophil Swarming against Live Microbial Targets Alex Hopke and Daniel Irimia 9. Micro-Injection and Micropipette Controlled Phagocytosis Methods for Neutrophils Maurice B. Hallett, Jennie S. Campbell, Iraj Laffafian, and Sharon Dewitt 10. Using Imaging Flow Cytometry to Quantify Neutrophil Phagocytosis Asya Smirnov, Michael D. Solga, Joanne Lannigan, and Alison K. Criss 11. Visualization and Quantification of Phagocytosis by Neutrophils Gaelen Guzman and Fikadu G. Tafesse 12. Analysis of Neutrophil Bactericidal Activity Nicholas J. Magon, Heather A. Parker, Louisa V. Ashby, Reuben J. Springer, and Mark B. Hampton Part IV: Biochemistry, Biology, and Signal Transduction of Neutrophils 13. Assessment of Neutrophil Apoptosis Nicole D. Barth, Marc Vendrell, David A. Dorward, Adriano G. Rossi, and Ian Dransfield 14. Optical Methods for the Measurement and Manipulation of Cytosolic Calcium Signals in Neutrophils Maurice B. Hallett, Rhiannon E. Roberts, and Sharon Dewitt 15. Labeling Acidic Compartments of Neutrophils with Cresyl Violet Philip P. Ostrowski, Ziv Roth, and Sergio Grinstein 16. Neutrophil Degranulation of Azurophil and Specific Granules Samia Bedouhene, Pham My-Chan Dang, Margarita Hurtado-Nedelec, and Jamel El-Benna 17. Influence of Oxygen on Function and Cholesterol Composition of Murine Bone Marrow-Derived Neutrophils Katja Branitzki-Heinemann, Graham Brogden, and Maren von Koeckritz-Blickwede 18. In Vitro Assay for Sensitive Determination of Human Blood PMN Responses Noah Fine, William Khoury, and Michael Glogauer 19. Fast and Accurate Quantitative Analysis of Cytokine Gene Expression in Human Neutrophils by Reverse Transcription Real-Time PCR Nicola Tamassia, Marco A. Cassatella, and Flavia Bazzoni 20. Detection of Intact Transcription Factors in Human Neutrophils Patrick P. McDonald and Richard D. Ye 21. Genome-Scale Transcript Analyses of Human Neutrophils Scott D. Kobayashi, Adeline R. Porter, Sarah L. Anzick, Dan E. Sturdevant, and Frank R. DeLeo Part V: NADPH Oxidase and Production of Reactive Oxygen Species 22. Measurement of Respiratory Burst Products, Released or Retained, During Activation of Professional Phagocytes Claes Dahlgren, Halla Bjoernsdottir, Martina Sundqvist, Karin Christenson, and Johan Bylund 23. Cell-Free NADPH Oxidase Activation Assays: A Triumph of Reductionism Edgar Pick Part VI: Analysis of Neutrophil Extracellular Traps 24. Immunofluorescent Detection of NET Components in Paraffin-Embedded Tissue Ulrike Abu-Abed and Volker Brinkmann 25. Detection, Visualization, and Quantification of Neutrophil Extracellular Traps (NETs) and NET Markers Nicole de Buhr and Maren von Koeckritz-Blickwede 26. Imaging of Neutrophils and Neutrophil Extracellular Traps (Nets) with Intravital (In Vivo) Microscopy Iwona Cichon, Michal Santocki, Weronika Ortmann, and Elzbieta Kolaczkowska

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