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Full Product Details

Author:   Tanja Zigova ,  J. Sanchez ,  Tanja Zigova ,  J. Sanchez
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Volume:   v. 198
Dimensions:   Width: 15.20cm , Height: 2.50cm , Length: 22.90cm
Weight:   0.794kg
ISBN:  

9780896039643

ISBN 10:   0896039641
Pages:   400
Publication Date:   28 February 2002
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Out of Print
Availability:   In Print  
Limited stock is available. It will be ordered for you and shipped pending supplier's limited stock.

Table of Contents

Part I. Isolation and Culture of NSCs Neural Differentiation of Embryonic Stem Cells K. Sue O'Shea Production and Analysis of Neurospheres from Acutely Dissociated and Postmortem CNS Specimens Eric D. Laywell, Valery G. Kukekov, Oleg Suslov, Tong Zheng, and Dennis A. Steindler Isolation of Stem and Precursor Cells from Fetal Tissue Yuan Y. Wu, Tahmina Mujtaba, and Mahendra S. Rao Olfactory Ensheathing Cells: Isolation and Culture from the Rat Olfactory Bulb Susan C. Barnett and A. Jane Roskams Culturing Olfactory Ensheathing Glia from the Mouse Olfactory Epithelium Edmund Au and A. Jane Roskams Production of Immortalized Human Neural Crest Stem Cells Seung U. Kim, Eiji Nakagawa, Kozo Hatori, Atsushi Nagai, Myung A. Lee, and Jung H. Bang Adult Rodent Spinal Cord Derived Neural Stem Cells: Isolation and Characterization Lamya S. Shihabuddin Preparation of Neural Progenitors from Bone Marrow and Umbilical Cord Blood Shijie Song and J. Sanchez-Ramos Seeding Neural Stem Cells on Scaffolds of PGA, PLA, and Their Copolymers Erin Lavik, Yang D. Teng, Evan Snyder, and Robert Langer Part II. Characterization of NSCs In Vitro A. Cellular Techniques Analysis of Cell Generation in the Telencephalic Neuroepithelium Takao Takahashi, Verne S. Caviness, Jr., and Pradeep G. Bhide Clonal Analyses and Cryopreservation of Neural Stem Cell Cultures Angelo L. Vescovi, Rossella Galli, and Angela Gritti Assessing the Involvement of Telomerase in Stem Cell Biology Mark P. Mattson, Peisu Zhang, and Weiming Fu Detection of Telomerase Activity in Neural Cells Karen R. Prowse In Vitro Assays for Neural Stem Cell Differentiation Marcel M. Daadi Electron Microscopy and Lac-Z Labeling Bela Kosaras and Evan Snyder B. Electrophysiological Techniques Techniques for Studying the Electrophysiology of Neurons Derived from Neural Stem/Progenitor Cells David S. K. Magnuson and Dante J. Morassutti C. Molecular Techniques Fluorescence In Situ Hybridization Barbara A. Tate and Rachel L. Ostroff RT-PCR Analyses of Differential Gene Expression in ES-Derived Neural Stem Cells Theresa E. Gratsch Differential Display: Isolation of Novel Genes Theresa E. Gratsch Cell Labeling and Gene Misexpression by Electroporation Terence J. Van Raay and Michael R. Stark Gene Therapy Using Neural Stem Cells Luciano Conti and Elena Cattaneo Modeling Brain Pathologies Using Neural Stem Cells Simonetta Sipione and Elena Cattaneo Part III. Utilization/Characterization of NSCs In Vivo A. Endogenous Pools of Stem/Progenitor Cells Activation and Differentiation of Endogenous Neural Stem Cell Progeny in the Rat Parkinson Animal Model Marcel M. Daadi Identification of Musashi1-Positive Cells in Human Normal and Neoplastic Neuroepithelial Tissues by Immunohistochemical Methods Yonehiro Kanemura, Shin-ichi Sakakibara, and Hideyuki Okano Identification of Newborn Cells by BrdU Labeling and Immunocytochemistry In Vivo Sanjay S. P. Magavi and Jeffrey D. Macklis Immunocytochemical Analysis of Neuronal Differentiation Sanjay S. P. Magavi and Jeffrey D. Macklis Neuroanatomical Tracing of Neuronal Projections with Fluoro-Gold Lisa A. Catapano, Sanjay S. P. Magavi, and Jeffrey D. Macklis B. Transplantation Labeling Stem Cells In Vitro for Identification of Their Differentiated Phenotypes After Grafting into the CNS Qi-lin Cao, Stephen M. Onifer, and Scott R. Whittemore Optimizing Stem Cell Grafting into the CNS Scott R. Whittemore, Y. Ping Zhang, Christopher B. Shields, Dante J. Morassutti, and David S. K. Magnuson Vision-Guided Technique for Cell Transplantation and Injection of Active Molecules into Rat and Mouse Embryos Lorenzo Magrassi Transplantation into Neonatal Rat Brain as a Tool to Study Properties of Stem Cells Tanja Zigova and Mary B. Newman Routes of Stem Cell Administration in the Adult Rodent Alison E. Willing, Svitlana Garbuzova-Davis Paul R. Sanberg, and Samuel Saporta

Reviews

...the book will be of value to stem cell researchers, as the protocols are presented in sufficient detail to make the replication of results likely between laboratories. Neural Stem Cells is a competently collected and presented set of methods. - Cell Therapy News

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