Overview
This updated edition collects cutting-edge techniques used to study neural stem and progenitor cells as well as the brain microenvironment. Featuring a wide range of technological advances in the study of neural stem cells, the volume highlights the promises of stem cell-based therapeutic applications for central nervous system ailments. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Neural Progenitor Cells: Methods and Protocols, Second Edition serves as an invaluable resource for the next generation of neuroscientists as they develop innovative experimental paradigms and progress toward therapeutic applications in the field of neurobiology.
Full Product Details
Author: Loic P. Deleyrolle
Publisher: Springer-Verlag New York Inc.
Imprint: Springer-Verlag New York Inc.
Edition: 2nd ed. 2022
Volume: 2389
Weight: 0.456kg
ISBN: 9781071617854
ISBN 10: 1071617850
Pages: 223
Publication Date: 25 September 2022
Audience:
Professional and scholarly
,
Professional & Vocational
Format: Paperback
Publisher's Status: Active
Availability: Manufactured on demand 
We will order this item for you from a manufactured on demand supplier.
Table of Contents
1. Culturing Mouse Fetal Neural Precursor Cells in a Free-Floating Serum-Free Condition Hassan Azari 2. Isolation and Culture of Neural Stem/Progenitor Cells from the Postnatal Periventricular Region Alessandra Ricca, Federica Cascino, and Angela Gritti 3. Isolation and Culture of Adult Hippocampal Precursor Cells as Free-Floating Neurospheres Ruslan Rust and Tara L. Walker 4. Isolate and Culture Neural Stem Cells from the Mouse Adult Spinal Cord Jean-Philippe Hugnot 5. Culturing and Expansion of “Clinical Grade” Neural Stem Cells from the Fetal Human Central Nervous System Maurizio Gelati, Daniela Celeste Profico, Daniela Ferrari, and Angelo Luigi Vescovi 6. Microglia Isolation from Neural Stem Cell-Enriched Regions Gregory P. Marshall II 7. Microglial FACS Sorting for Robust RNA Recovery for Next Generation Sequencing Andria L. Doty and Aline Oliveria 8. Method for Isolating Extracellular Vesicles from Human Neural Stem Cells Expanded under Neurosphere Culture Nasser Nassiri Koopaei, Thomas D. Schmittgen, Brent A. Reynolds, and Hassan Azari 9. Isolating and Culturing of Precursor Cells from the Adult Human Brain Florian A. Siebzehnrubl 10. Isolation and Culture of Precursor Cells from the Adult Human Spinal Cord Luc Bauchet, Nicolas Lonjon, Florence Vachiery-Lahaye, Alain Boularan, Alain Privat, and Jean-Philippe Hugnot 11. Isolation and Enrichment of Defined Neural Cell Populations from Heterogeneous Neural Stem Cell Progeny Hassan Azari 12. Identifying Neural Progenitor Cells in the Adult Human Brain Thomas I.H. Park, Henry J. Waldvogel, Johanna M. Montgomery, Edward W. Mee, Peter S. Bergin, Richard L.M. Faull, Mike Dragunow, and Maurice A. Curtis 13. Neonatal Transplant in Hypoxic Injury Tong Zheng and Michael D. Weiss 14. Isolation and Purification of Self Renewable Human Neural Stem Cells from iPSCs for Cell Therapy in Experimental Model of Ischemic Stroke Marcel M. Daadi 15. Generating Cerebral Organoids from Human Pluripotent Stem Cells Leon Chew, Adam Añonuevo, and Erin Knock 16. Advancing Our Understanding of Brain Disorders: Research Using Postmortem Brain Tissue Maurice Curtis and Vinata Vedam-Mai 17. Biovalue in Human Brain Banking: Applications and Challenges for Research in Neurodegenerative Diseases Vinata Vedam-Mai
Reviews
Author Information
Tab Content 6
Author Website:
Customer Reviews
Recent Reviews
No review item found!
Add your own review!
Countries Available
All regions
|
