Overview

Cell death is an essential process in development, and a major contributor to a wide range of human diseases.  Three major classifications of cell death, apoptosis, autophagic cell death and necrosis, have been described for years, and the existence of many more forms of cell death is now accepted. In, Necrosis: Methods and Protocols experts in the field provide a wide range of methods and techniques for the study of necrosis in vitro and in vivo. These include methods and techniques for the analysis of necrosis in mammalian cells, characterization of alternative forms of cell death: entosis and pyroptosis, and analysis of cell death in non-mammalian model sytems and mammalian tissues, including chapters on skin, brain, and heart. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls.     Authoritative and practical, Necrosis: Methods and Protocols describes techniques  in an easy to follow manner, with details so that beginners can succeed with challenging techniques.

Full Product Details

Author:   Kimberly McCall ,  Charles Klein
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2013
Volume:   1004
Dimensions:   Width: 17.80cm , Height: 1.40cm , Length: 25.40cm
Weight:   5.091kg
ISBN:  

9781493960101

ISBN 10:   1493960105
Pages:   252
Publication Date:   23 August 2016
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Navigation to the Graveyard- Induction of Various Pathways of Necrosis and their Classification by Flow Cytometry.- Time-lapse Imaging of Necrosis.- Activity Assays for Receptor Interacting Protein Kinase 1: A Key Regulator of Necroptosis.- Fluorescent Biosensors for the Detection of HMGB1 Release.- Methods for the Study of Entosis.- Analysis of Pyroptosis in Bacterial Infection.- Analysis of Cell Death by Electron Microscopy.- Histological Analysis of Neurodegeneration in the Mouse Brain.- Quantitation of Acute Necrosis after Experimental Myocardial Infarction.- Immunohistological Tools to Discriminate Apoptotic and Necrotic Cell Death in the Skin.- Identification and Characterization of Programmed Cell Death Markers in Bacterial Models.- Quantification of Genetically Controlled Cell Death in Budding Yeast.- Necrosis in C. elegans.- Monitoring the Clearance of Apoptotic and Necrotic Cells in the Nematode Caenorhabditis elegans.- Modeling Calcium-overload Mediated Necrosis in Drosophila.- Use of Necrotic Markers in the Drosophila Ovary.- Detection and Measurement of Necrosis in Plants.

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