Overview

This detailed book provides a broad collection of methodologies for natural killer (NK) cell research. Beginning with an assortment of methods for the isolation of NK cells and NK cell differentiation, the volume continues with methodologies for functional tests, such as cytotoxicity, viral infection, and metabolism assays, as well as clinical applications of NK cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Natural Killer (NK) Cells: Methods and Protocols serves as an ideal guide for researchers looking to contribute to the further development of basic and clinical NK cell research.

Full Product Details

Author:   Noriko Shimasaki
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2022
Volume:   2463
Weight:   0.655kg
ISBN:  

9781071621622

ISBN 10:   1071621629
Pages:   330
Publication Date:   30 March 2023
Audience:   College/higher education ,  Professional and scholarly ,  Postgraduate, Research & Scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I: NK Cell Specification   1. Isolation of Innate Lymphoid Cells from Murine Intestinal Lamina Propria             Ao Mei, Elaheh Hashemi, Mohamed Khalil, Dandan Wang, and Subramaniam Malarkannan   2. Purification of Primary Decidual Natural Killer Cells for Functional Analysis             Ângela C. Crespo, Aria Alexander, and Tamara Tilburgs   3. In Vitro Development of Mouse and Human NK Cells from Hematopoietic Progenitor Cells             Ines Ullmo, Nahide Koksal, Heather Y.K. Ang, and Hugh J.M. Brady   4. Induction of Human Natural Killer Cells under Defined Conditions by Seamless Transition from Maintenance Culture of Pluripotent Stem Cells             Akira Niwa and Megumu K. Saito   5. Development of Humanized Mouse Models for Studying Human NK Cells in Health and Disease             Liang Shan, Richard A. Flavell, and Dietmar Herndler-Brandstetter   6. Antibody-Oligonucleotide Conjugation Using a SPAAC Copper-Free Method Compatible with 10x Genomics’ Single-Cell RNA-Seq Dominic Paul Lee, Wang Jiehao Ray, Tan Pee Mei, Shawn Hoon, Jonathan Scolnick, and Gene W. Yeo   7. Methods to Analyze the Developmental Trajectory of Human Primary NK Cells Using Monocle and SCENIC Analyses             Dandan Wang, Robert Burns, Mohamed Khalil, Ao Mei, Elaheh Hashemi, and Subramaniam Malarkannan   8. Methods for Isolating and Defining Single-Cell Transcriptomes of Tissue-Resident Human NK Cells             Elaheh Hashemi, Ao Mei, Dandan Wang, Mohamed Khalil, and Subramaniam Malarkannan   9. Retrogenic Color-Barcoding for Fate Mapping of Single Innate Lymphocytes             Simon Grassmann, Joseph C. Sun, and Veit R. Buchholz   10. Quantifying Human Natural Killer Cell Migration by Imaging and Image Analysis             Amera L. Martinez, Michael J. Shannon, Shira E. Eisman, Everardo Hegewisch-Solloa, Aneeza N. Asif, Tasneem A.M. Ebrahim, and Emily M. Mace   11. Tracking of NK Cells by Positron Emission Tomography Using 89Zr-Oxine Ex Vivo Cell Labeling             Noriko Sato, Lawrence P. Szajek, and Peter L. Choyke   Part II: NK Cell Functional Assays   12. Assessing Changes in Human Natural Killer Cell Metabolism Using the Seahorse Extracellular Flux Analyzer             Javier Traba and Olga M. Antón   13. Determining Activation Status of Natural Killer Cells Following Stimulation via Cytokines and Surface Receptors             Lizeth G. Meza Guzman and Sandra E. Nicholson   14. Method to Study Adaptive NK Cells Following MCMV Infections             Mohamed Khalil, Ao Mei, Elaheh Hashemi, Dandan Wang, Megan Schumacher, Scott Terhune, and Subramaniam Malarkannan   15. Assessing the Response of Human NK Cell Subsets to Infection by Clinically-Isolated Virus Strains             Nobuyo Yawata and Makoto Yawata   16. NK Cell Isolation and Cytotoxicity by Radioactive Chromium Release Assay and DELFIA -EuTDA Cytotoxicity Assay             Casey W. Buller and Stephen O. Mathew   17. Organoid Co-Culture Methods to Capture Cancer Cell -Natural Killer Cell Interactions             Isaac S. Chan and Andrew J. Ewald   18. In Vitro Visualization of Cell-to-Cell Interactions between Natural Killer Cells and Sensory Neurons             Hyoung Woo Kim, Alexander J. Davies, and Seog Bae Oh   19. CRISPR Screen to Identify Factors that Render Tumor Cells Sensitive or Resistant to Killing by NK Cells             Xiaoxuan Zhuang and Eric O. Long   Part III: Clinical Application   20. Practical Considerations and Workflow in Utilizing KIR Genotyping in Transplantation Medicine             Makoto Yawata and Nobuyo Yawata   21. Gene Transduction of Natural Killer Cells for Clinical Application             Noriko Shimasaki

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