Overview

This volume provides comprehensive dry and wet experiments, methods, and applications on nanopore sequencing. Chapters guide readers through bioinformatic procedures, genome sequencing, analysis of repetitive regions, structural variations, rapid and on-site microbial identification, epidemiology, and transcriptome analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and methods, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.   Authoritative and cutting-edge, Nanopore Sequencing: Methods and Protocols aims to be comprehensive guide for researchers.

Full Product Details

Author:   Kazuharu Arakawa
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2023
Volume:   2632
Weight:   0.831kg
ISBN:  

9781071629956

ISBN 10:   1071629956
Pages:   324
Publication Date:   15 February 2023
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I. Nanopore sequencing for genomics and beyond 1. The current state of nanopore sequencing Jonathan Pugh 2. Hybrid genome assembly of short and long reads in Galaxy Tazro Ohta and Yuh Shiwa   3. Microbial genome sequencing and assembly using nanopore sequencers, Makoto Taniguchi and Kazuma Uesaka   4. De novo genome assembly of Japanese Black cattle as model of an economically relevant animal Shinji Sasaki, Yasuhiko Haga, Hiroyuki Wakaguri, Kazumi Abe, and Yutaka Suzuki   5. How to sequence and assemble plant genomes Ken Naito   6. Detection of DNA modification using nanopore sequencers Yoshikazu Furuta   7. Ultra-low input genome library preparation for nanopore sequencing with droplet-MDA Kazuharu Arakawa   8. The method of eliminating the Wolbachia endosymbiont genomes from insect samples prior to long-reads Keizo Takasuka   9. A nanopore sequencing course for graduate school curriculum Kazuharu Arakawa   Part II. Analysis of repetitive regions and structural variants   10. A guide to genome sequencing containing ultra-long repetitive regions Nobuaki Kono   11. Analysis of tandem repeat expansions using long DNA reads Satomi Mitsuhashi and Martin C Frith   12. Finding rearrangements in nanopore DNA reads with last and dnarrange Martin C. Frith and Satomi Mitsuhashi   13. Long read whole-genome sequencing using a nanopore sequencer and detection of structural variants in cancer genomes Yasuhiko Haga, Yoshitaka Sakamoto, Miyuki Arai, Yutaka Suzuki and Ayako Suzuki   Part III. Rapid on-site microbial detection and epidemiology   14. Full-length 16S rRNA gene analysis using long-read nanopore sequencing for rapid identification of bacteria from clinical specimens Yoshiyuki Matsuo   15.Nanopore sequencing data analysis of 16S rRNA genes using Genome Sync-GSTK system Kirill Kryukov, Tadashi Imanishi and So Nakagawa   16. Genomic epidemiological analysis of antimicrobial-resistant bacteria with nanopore sequencing Masato Suzuki, Yusuke Hashimoto, Aki Hirabayashi, Koji Yahara, Mitsunori Yoshida, Hanako Fukano, Yoshihiko Hoshino, Keigo Shibayama, Haruyoshi Tomita   17. Rapid and comprehensive identification of nontuberculous mycobacteria, Yuki Matsumoto and Shota Nakamura     Part IV. Nanopore sequencing for transcriptomics and beyond   18. Long-read single-cell sequencing using scCOLOR-seq Martin Philpott, Udo Oppermann and Adam P Cribbs   19. Unfolding the Bacterial Transcriptome Landscape using Oxford Nanopore Technology Direct RNA Sequencing Mohamad Al kadi and Daisuke Okuzaki   20. Nanopore Direct RNA Sequencing of Monosome-and Polysome-bound RNA Lan Anh Nguyen, Toshifumi Inada, and Josephine Galipon   21. RNA modification detection using nanopore direct RNA sequencing and nanoDoc2 Hiroki Ueda, Bhaskar Dasgupta, Bo-yi Yu

Reviews

Author Information

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions