Mycobacteria Protocols

Author:   Tanya Parish ,  David M. Roberts
Publisher:   Humana Press Inc.
Edition:   Softcover reprint of the original 3rd ed. 2015
Volume:   1285
ISBN:  

9781493955824


Pages:   416
Publication Date:   05 October 2016
Format:   Paperback
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

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Mycobacteria Protocols


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Author:   Tanya Parish ,  David M. Roberts
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 3rd ed. 2015
Volume:   1285
Weight:   8.956kg
ISBN:  

9781493955824


ISBN 10:   1493955829
Pages:   416
Publication Date:   05 October 2016
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Whole Genome Sequencing for Comparative Genomics and De Novo Genome Assembly.- Whole Transcriptome Sequencing for High-resolution Transcriptomic Analysis in Mycobacterium tuberculosis.- RNA Sequencing for Transcript 5’-end Mapping in Mycobacteria.- Fractionation and Analysis of Mycobacterial Proteins.- Lipid and Lipoarabinomannan Isolation and Characterization.- Metabolomics of Mycobacterium tuberculosis.- Electroporation of Mycobacteria.- Targeted Gene Knockout and Essentiality Testing by Homologous Recombination.- Construction of Conditional Knockdown Mutants in Mycobacteria.- Mycobacterial Recombineering.- In vitro Models that Utilize Hypoxia to Induce Non-replicating Persistence in Mycobacteria.- Genetic Dissection of Mycobacterial Biofilms.- Measuring Efflux and Permeability in Mycobacteria.- Single-cell Analysis of Mycobacteria using Microfluidics and Time-lapse Microscopy.- Antimicrobial Susceptibility Testing for Mycobacterium sp..- Determination of Compound Kill Kinetics Against Mycobacterium tuberculosis.- Microplate Alamar Blue Assay (MABA) and Low Oxygen Recovery Assay (LORA) for Mycobacterium tuberculosis.- A Multi-Stress Model for High Throughput Screening Against Non-replicating Mycobacterium tuberculosis.- Isolation and Characterization of Compound-resistant Isolates of Mycobacterium tuberculosis.- Macrophage Infection Models for Mycobacterium tuberculosis.- Infection of Human Neutrophils to Study Virulence Properties of Mycobacteriumtuberculosis.- Isolation of Bead-phagosomes to Study Virulence Function of M. tuberculosis Cell Wall Lipids.- Live Imaging of Mycobacterium marinum Infection in Dictyostelium discoideum.- Testing Chemical and Genetic Modulators in Mycobacterium tuberculosis Infected Cells using Phenotypic Assays.  

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