Overview

This volume provides a collection of state-of-the-art approaches addressing key aspects of multiplexed imaging. Chapters focus on labeling and imaging techniques for multiplexed imaging, as well as on the application of these techniques for the study of cells and tissues. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and practical, Multiplexed Imaging: Methods and Protocols aims to be helpful for researchers interested in implementing multiplexed imaging or in developing novel, cutting-edge multiplexed imaging approaches.

Full Product Details

Author:   Eli Zamir
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2021
Volume:   2350
Weight:   0.684kg
ISBN:  

9781071615959

ISBN 10:   1071615955
Pages:   343
Publication Date:   31 July 2021
Audience:   College/higher education ,  Postgraduate, Research & Scholarly
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1.     Strategies for Multiplexed Biosensor Imaging to Study Intracellular Signaling Networks Jeremiah Keyes, Sohum Mehta, and Jin Zhang   2.     Six-color Confocal Immunofluorescence Microscopy with 4-Laser Lines Lukas Heger, Jennifer J. Lühr, Lukas Amon, Ana-Sunčana Smith, Nathalie Eissing, and Diana Dudziak   3.     Multiplexed Imaging of Posttranslational Modifications of Endogenous Proteins in Live Cells Yuko Sato and Hiroshi Kimura   4.     Multiplex Imaging of Rho GTPase Activities in Living Cells Ravi M. Bhalla, Maren Hülsemann, Polina V. Verkhusha, Myla G. Walker, Daria Shcherbakova, and Louis Hodgson   5.     Multicolor Localization-Based Super Resolution Microscopy Leila Nahidiazar and Rolf Harkes   6.     Multiplexed Tissue Tomography Evan H. Phillips, David Scholten, Amy C. Flor, Stephen J. Kron, and Steve Seung-Young Lee   7.     Multicolor 3D Confocal Imaging of Thick Tissue Sections Leo Kunz and Daniel L. Coutu   8.     Multiphoton Deep-Tissue Imaging of Micrometastases and Disseminated Cancer Cells Using Conjugates of Quantum Dots and Single-Domain Antibodies Alyona Sukhanova, Fernanda Ramos-Gomes, Patrick Chames, Pavel Sokolov, Daniel Baty, Frauke Alves, and Igor Nabiev   9.     Multiplexed Imaging for immune profiling on Human FFPE Material Artur Mezheyeuski and Carina Strell   10.  Method for Multiplexed Dynamic Intravital Multi-Photon Imaging Asylkhan Rakhymzhan, Andreas Acs, Ruth Leben, Thomas H. Winkler, Anja E. Hauser, and Raluca A. Niesner   11.  Fourier Multiplexed Fluorescence Lifetime Imaging Leilei Peng   12.  Bimolecular Fluorescence Complementation (BiFC) and Multiplexed Imaging of Protein-Protein Interactions in Human Living Cells Yunlong Jia, Françoise Bleicher, Jonathan Reboulet, and Samir Merabet   13.  Out-of-Phase Imaging after Optical Modulation (OPIOM) for Multiplexed Fluorescence Imaging under Adverse Optical Conditions Raja Chouket, Ruikang Zhang, Agnès Pellissier-Tanon, Annie Lemarchand, Agathe Espagne, Thomas Le Saux, and Ludovic Jullien   14.  Multicolor Bioluminescence Imaging of Subcellular Structures and Multicolor Calcium Imaging in Single Living Cells Kazushi Suzuki, Md Nadim Hossain, Tomoki Matsuda, and Takeharu Nagai   15.  Nanoparticles for In Vivo Lifetime Multiplexed Imaging Erving Ximendes, Emma Martín Rodríguez, Dirk Ortgies, Meiling Tan, Guanying Chen, and Blanca del Rosal   16.  Versatile on-Demand Fluorescent Labeling of Fusion Proteins using Fluorescence-Activating and Absorption-Shifting Tag (FAST)  Arnaud Gautier, Ludovic Jullien, Chenge Li, Marie-Aude Plamont, Alison G. Tebo, Marion Thauvin, Michel Volovitch, and Sophie Vriz   17.  UltraPlex Hapten-Based Multiplexed Fluorescent Immunohistochemistry Matt Levin, Amy Flor, Helen Snyder, Stephen J. Kron, and David Schwartz   18.  Multi-Modal Approach for Cancer Cells Investigation Alexandre Berquand and Jerôme Devy   19.  Multiplexed Fourier Transform Infrared and Raman Imaging Guillermo Quintás, Bayden R. Wood, Hugh J. Byrne, and David Perez-Guaita   20.  Multiplexed Imaging Mass Spectrometry of Histological Staining, N-glycan and Extracellular Matrix from One Tissue Section: A Tool for Fibrosis Research Cassandra L. Clift, Anand Mehta, Richard R. Drake, and Peggi M. Angel   21.  Multiplexed Raman Imaging in Tissues and Living Organisms Travis M Shaffer and Sanjiv S. Gambhir

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