Morphology Methods: Cell and Molecular Biology Techniques

Author:   Ricardo V. Lloyd
Publisher:   Humana Press Inc.
Edition:   Softcover reprint of hardcover 1st ed. 2001
ISBN:  

9781617372735


Pages:   422
Publication Date:   09 December 2010
Format:   Paperback
Availability:   Out of stock   Availability explained
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Morphology Methods: Cell and Molecular Biology Techniques


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Overview

Ricardo Lloyd, md, phd, provides critical and tutorial surveys of the cutting-edge molecular and cell biologic techniques used today in the analysis of cells and tissues in situ. These molecular-based histologic and morphologic techniques are described in step-by-step detail by hands-on experts, often those who have originated or perfected them. Among the techniques presented are laser capture microdissection, in situ hybridization, FISH, confocal laser scanning microscopy, in situ PCR, and tyramide amplification. Applications include the detection of infectious agents and DNA, the diagnosis of undifferentiated tumors and endocrine lesions, the metaphase analysis of whole blood cultures, and clonality analysis. In its integrated, state-of-the-art approach, Morphology Methods: Cell and Molecular Biology Techniques thoroughly details and applies the rich panoply of significant new cell and molecular biologic technologies to all the major problems confronting morphologic studies and diagnostic pathology today.

Full Product Details

Author:   Ricardo V. Lloyd
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of hardcover 1st ed. 2001
Dimensions:   Width: 17.80cm , Height: 2.30cm , Length: 25.40cm
Weight:   0.846kg
ISBN:  

9781617372735


ISBN 10:   1617372730
Pages:   422
Publication Date:   09 December 2010
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Out of stock   Availability explained
The supplier is temporarily out of stock of this item. It will be ordered for you on backorder and shipped when it becomes available.

Table of Contents

Introduction to Molecular Methods Ricardo V. Lloyd Laser Capture Microdissection: Principles and Applications Ricardo V. Lloyd (Protocols: H&E Staining, Operating the PIX Cell System; DNA Extraction from Collected Cells; RNA Extraction from Collected Cells) In Situ Hybridization: Detection of DNA and RNA Long Jin, Xiang Qian, and Ricardo V. Lloyd (Protocol: In Situ Hybridization) In Situ Detection of Infectious Agents Gary W. Procop and Randall Hayden (Protocols: Detecting Adenovirus, Epstein-Barr Virus) Fluorescent In Situ Hybridization (FISH) Susan Sheldon (Protocol: FISH Materials) Practical Applications of the FISH Technique George Kontogeorgos, Nikiforos Kapranos, and Eleni Thodou (Protocols: Metaphase Analysis from Whole Blood Culture; Metaphase Analysis from Solid Tumors; Interphase Analysis on Cell Imprints-Cytology Specimens; Interphase Analysis of Formalin-Fixed Paraffin-Embedded Tissues) Tyramide Amplification Methods for In Situ Hybridization John L. Frater and Raymond R. Tubbs (Protocol: TSA, 1995; CARD/TSA with Nanogold; Tyramide-enhanced FISH with Peroxidase-labeled Oligonucleotides) Protocols: Ultrastructural In Situ Hybridization Akira Matsuno, Tadashi Nagashima, R. Yoshiyuki Osamura, and Keiichi Watanabe Quantitation of In Situ Hybridization Analysis Lars-Inge Larsson Protocols: Confocal Laser Scanning Microscopy Akira Matsuno, Johbu Itoh, Tadashi Nagashima, R. Yoshiyuki Osamura, and Keiichi Watanabe Protocols: Polymerase Chain Reaction Yuri E. Nikiforov and Philip N. Howles Protocols: Detection of Nucleic Acids in Cells and Tissues by In Situ Polymerase Chain Reaction Omar Bagasra, Lisa E. Bobroski, and Muhammad Amjad Immunohistochemistry: Theory and Practice Patrick C. Roche and Eric D. His Antigen Retrieval Technique for Immunohistochemistry: Principles, Protocols, and Further Development Clive R. Taylor and Shan-Rong Shi (Protocol: Antigen Retrieval (AR) Technique) Tyramide Amplification in Immunohistochemistry Naoko Sanno, Akira Teramoto, and R. Yoshiyuki Osamura (Protocol: CARD Immunohistochemistry) Application of Immunohistochemistry in the Diagnosis of Lymphoid Lesions Paul J. Kurtin Applications of Immunohistochemistry in the Diagnosis of Undifferentiated Tumors Mark R. Wick and Lisa A Cerilli. Applications of Immunohistochemistry in the Diagnosis of Endocrine Lesions Ricardo V. Lloyd Ultrastructural Immunohistochemistry Sergio Vidal, Eva Horvath, and Kalman Kovacs (Protocol: Ultrastructural Immunohistochemistry) Protocols: Clonality Analysis, Aurel Perren and Paul Komminoth

Reviews

The book nicely covers the hot topics in this area -- laser capture microdissection, in situ hybridization, FISH, tyramide amplification methods, etc. An attractive feature of this book is that the last 10 chapters (of the 20) contain actual cookbook protocols (e.g., PCR, confocal laser scanning microscopy, immunohistochemistry, antigen retrieval, etc.) that can be adapted immediately to any laboratory...This book would be useful for those needing a concise reference for how to perform modern molecular tissue-based technology. -Doody's Health Science Book Review Journal This book describes in detail new methods employed in the study of molecular morphology as a basis for the study of human genes and the etiology of diseases. Among the methods are in situ hybridization, in situ detection of infectious agents, the FISH technique, PCR, immunocytochemistry, etc. This excellent production has many photos, some in color, and tables and figures. Very useful to those wishing to employ these techniques. - Journal of Pediatric Endocrinology and Metabolism


"""The book nicely covers the hot topics in this area -- laser capture microdissection, in situ hybridization, FISH, tyramide amplification methods, etc. An attractive feature of this book is that the last 10 chapters (of the 20) contain actual ""cookbook"" protocols (e.g., PCR, confocal laser scanning microscopy, immunohistochemistry, antigen retrieval, etc.) that can be adapted immediately to any laboratory...This book would be useful for those needing a concise reference for how to perform modern molecular tissue-based technology. "" -Doody's Health Science Book Review Journal ""This book describes in detail new methods employed in the study of molecular morphology as a basis for the study of human genes and the etiology of diseases. Among the methods are in situ hybridization, in situ detection of infectious agents, the ""FISH"" technique, PCR, immunocytochemistry, etc. This excellent production has many photos, some in color, and tables and figures. Very useful to those wishing to employ these techniques."" - Journal of Pediatric Endocrinology and Metabolism"


The book nicely covers the hot topics in this area -- laser capture microdissection, in situ hybridization, FISH, tyramide amplification methods, etc. An attractive feature of this book is that the last 10 chapters (of the 20) contain actual cookbook protocols (e.g., PCR, confocal laser scanning microscopy, immunohistochemistry, antigen retrieval, etc.) that can be adapted immediately to any laboratory...This book would be useful for those needing a concise reference for how to perform modern molecular tissue-based technology. -Doody's Health Science Book Review Journal This book describes in detail new methods employed in the study of molecular morphology as a basis for the study of human genes and the etiology of diseases. Among the methods are in situ hybridization, in situ detection of infectious agents, the FISH technique, PCR, immunocytochemistry, etc. This excellent production has many photos, some in color, and tables and figures. Very useful to those wishing to employ these techniques. - Journal of Pediatric Endocrinology and Metabolism


The book nicely covers the hot topics in this area -- laser capture microdissection, in situ hybridization, FISH, tyramide amplification methods, etc. An attractive feature of this book is that the last 10 chapters (of the 20) contain actual cookbook protocols (e.g., PCR, confocal laser scanning microscopy, immunohistochemistry, antigen retrieval, etc.) that can be adapted immediately to any laboratory...This book would be useful for those needing a concise reference for how to perform modern molecular tissue-based technology. -Doody's Health Science Book Review Journal This book describes in detail new methods employed in the study of molecular morphology as a basis for the study of human genes and the etiology of diseases. Among the methods are in situ hybridization, in situ detection of infectious agents, the FISH technique, PCR, immunocytochemistry, etc. This excellent production has many photos, some in color, and tables and figures. Very useful to those wishing to employ these techniques. - Journal of Pediatric Endocrinology and Metabolism


The book nicely covers the hot topics in this area -- laser capture microdissection, in situ hybridization, FISH, tyramide amplification methods, etc. An attractive feature of this book is that the last 10 chapters (of the 20) contain actual cookbook protocols (e.g., PCR, confocal laser scanning microscopy, immunohistochemistry, antigen retrieval, etc.) that can be adapted immediately to any laboratory...This book would be useful for those needing a concise reference for how to perform modern molecular tissue-based technology. -Doody's Health Science Book Review Journal This book describes in detail new methods employed in the study of molecular morphology as a basis for the study of human genes and the etiology of diseases. Among the methods are in situ hybridization, in situ detection of infectious agents, the FISH technique, PCR, immunocytochemistry, etc. This excellent production has many photos, some in color, and tables and figures. Very useful to those wishing to employ these techniques. - Journal of Pediatric Endocrinology and Metabolism


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