Overview

Monoclonal antibodies have revolutionized immunology and are now promising to have a similar impact on clinical medicine. Recent developments should overcome many of the difficulties experienced in the past which has seen a great deal of talk about the th

Full Product Details

Author:   Heddy Zola
Publisher:   Taylor & Francis Ltd
Imprint:   Bios Scientific Publishers Ltd
Dimensions:   Width: 15.60cm , Height: 1.40cm , Length: 23.40cm
Weight:   0.498kg
ISBN:  

9781872748788

ISBN 10:   1872748783
Pages:   220
Publication Date:   26 June 1995
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Postgraduate, Research & Scholarly
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

"Contributors -- Abbreviations -- Preface -- Safety note -- 1. Introduction. H. Zola -- Introduction: the purpose of this book -- Monoclonal antibodies: scope and limitations -- Antibodies as laboratory reagents: a brief perspective -- Antibodies as therapeutic agents: a brief perspective -- Conclusions -- References -- 2. Making and using 'conventional' mouse monoclonal antibodies. P.J. Donohoe, P.J. Macardle and H. Zola -- Introduction -- Tissue culture -- Materials -- Working solutions -- The screening assay -- Immunization -- The myeloma cell line -- Fusion protocol -- Feeder cells -- Post-fusion care -- Mycoplasma -- Cloning -- Cryopreservation -- Thawing cells out -- Ascites fluid -- Production of monoclonal antibodies in dialysis tubing -- Continuous perfusion culture systems -- Purification -- Conjugation -- References -- 3. Human monoclonal antibodies from immortalized B cells. A. W. Hohmann, L. Spatz, M. Irigoyen and A. Manheimer-Lory -- Introduction -- Immortalized B cells to probe the human antibody repertoire -- Infectious diseases -- Human immunodeficiency virus -- Erythrocyte antigens -- Human histocompatibility antigens -- Tumor antigens -- Autoantibodies -- Immortalization of B cells with EBV -- EBV transformation of B cells: methodology -- Making human hybridomas -- Hybridoma methodology -- References -- 4. Reconstruction of monoclonal antibodies by genetic engineering. J.R. Adair and D.J. King -- Introduction -- The reconstruction process -- The binding site -- The effector functions -- Reconstructed antibodies in practice -- Summary and conclusions -- References -- 5. The genetic engineering of antibody constructs for diagnosis and therapy. G.A. Pietersz and l.F.C. McKenzie -- Introduction -- Principles of use of antibody conjugates -- Recent advances in immunoconjugate construction -- Recombinant antibodies and fusion proteins -- Use of cytokines and growth factors for targeting -- Potential problems -- Conclusions -- References -- 6. Superseding hybridoma technology with phage display libraries. R. Irving and P. Hudson -- Introduction -- Affinity maturation -- Discussion -- References -- 7. Construction and application of libraries of artificial antibodies. W. Ito and Y. Kurosawa -- Introduction -- Expression in E. coli of antibodies whose V genes are derived from V"" and V L genes expressed in animals -- Characteristics of phage display antibodies -- Various devices for the generation of artificial antibodies -- Construction of libraries of completely synthetic antibodies -- Application of libraries of artificial antibodies -- Future perspectives -- References -- 8. Expression of antibody genes in mammalian cells. C. Bebbington -- Introduction -- Transient expression -- Stable mammalian expression systems -- Other factors influencing expression in mammalian cells -- Choosing an expression system -- References -- 9. Structure and application of single-chain Fvs as diagnostic and therapeutic agents. P. Hudson -- Introduction -- Design of single-chain Fv fragments -- Construction of scFv genes -- The structure of scFv fragments -- The design of fusion proteins -- The design of bifunctional scFvs ( diabodies) -- The choice of expression systems -- Scale-up production of scFvs in fermenters -- Case studies: the design and expression of scFv molecules (for crystal structures and novel diagnostics) -- Applications of scFvs in diagnostics -- Discussion -- References -- Index."

Reviews

...a very clear account of the new approaches to generating human antibodies. It compares and contrasts conventional hybridoma technology with the more recent approaches, enabling the reader to assess the impact and usefulness of the different technique

Author Information

Chief Hospital Scientist and Professor of Immunology, Flinders Medical Centre and Flinders University, Adelaide, Australia.

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