Overview

Procedures for hydridoma generation as well as detailed test protocols for the characterization and application of monoclonal antibodies are featured in this manual designed especially for use at the lab bench. Immunization, cell preparation, hybridization, cell culture and the cloning of hybridomas, the prevention of back-mutation, purification, labelling and the characterization of the antibodies obtained are just a sample of keywords taken from the contents. Practical hints and detailed product information together with protocols for troubleshooting make it a useful reference as well as a compendium of immunological methods over and above monoclonal antibody technology. The authors have emphasized protocols which tend to minimize the number of animals used in experimentation. They also favour methods which avoid the use of radioactivity.

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9783540508434

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