Overview

Included are chapters on sampling design, collection and storage of tissues (a guide to major tissue collections has been added), each of the major molecule techniques, interspecific and phylogenetic analysis, and a review of applications. The technique chapters cover principles, assumptions, applications, limitations, and basic protocols for isozyme electrophoresis, molecular cytogenetics, DNA hybridization, the polymerase chain reaction (PCR), DNA fragments (including microsatellites and RAPDs), restriction sites analysis, cloning and nucleic acid sequencing. The chapters on analysis have been expanded to include the many new developments in techniques for analyzing molecular data, including an expanded discussion of maximum likelihood methods, methods for assessing the results, comparative methods, and models of molecular evolution.

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9780878932825

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