Overview

Molecular Plant Biology is an all-new replacement for the original Practical Approach book Plant Molecular Biology that was first published in 1988. The rapid advances made in plant sciences during the past decade are reflected by the need to produce a two-volume book to cover all the relevant methodologies. The new book incorporates many of the fundamental procedures outlined in the original book, but these are fully updated to reflect advances in technology and the development of new methodologies. The new books also incorporate many approaches that were not available in the earlier volume. Each chapter has been written by an international expert with current practical expertise in the topics covered. Each book is divided into three sections. The approaches outlined in Volume One cover a wide range of techniques for gene isolation, gene identification and subsequent gene isolation as well as for studies of gene organization. . The methods described range from classical mutagenesis through plant transformation, T-DNA and transposon tagging strategies, genomic subtraction, gene mapping, construction and screening of YAC, BAC and cosmid libraries chromosome in situ, and isolation of cDNA sequences by western and southwestern library screens, and complementation cloning.

Full Product Details

Author:   Philip M Gilmartin (Director, Centre for Plant Sciences, University of Leeds) ,  Chris Bowler (Head, Laboratory of Molecular Plant Biology, Stazione Zoologica, Napoli, Italy)
Publisher:   Oxford University Press
Imprint:   Oxford University Press
Volume:   258
Dimensions:   Width: 19.50cm , Height: 2.10cm , Length: 25.30cm
Weight:   0.800kg
ISBN:  

9780199638765

ISBN 10:   0199638764
Pages:   296
Publication Date:   18 April 2002
Audience:   College/higher education ,  Professional and scholarly ,  Undergraduate ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Out of Print
Availability:   Out of stock  

Table of Contents

Part 1: Gene Identification 1: Maarten Koornneef: Classical mutagenesis in higher plants 2: Karabi Datta and Swapan K Datta: Plant transfomrmation 3: Csaba Koncz and Jeff Schell: T-DNA tagging 4: Andy Pereira: Transposion tagging methods in heterologous plants 5: Tai-ping Sun: Cloning plant genes by genomic subtraction Part 2: Gene Organization 6: S Grandillo and T M Fulton: Approaches to gene mapping 7: Paul Muskett, Ian Bancroft and Caroline Dean: Arabidopsis YAC, BAC and cosmid libraries 8: J S Heslop-Harrison and Trude Schwarzacher: In situ hybridization to plant chromosomes and DNA fibres Part 3: Library Screening and cDNA Isolation 9: Michiel Vandenbussche and Tom Gerats: PCR cloning approaches 10: Rhonda C Foley and Karam B Singh: Western and southwestern library screens 11: E Ann Oakenfull and James A H Murray: Complementation Cloning

Reviews

This Practical Approach book will have a broad distribution and is essential on each plant molecular biology bench. Journal of Physiology

This Practical Approach book will have a broad distribution and is essential on each plant molecular biology bench. * Journal of Physiology *

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