Overview

Molecular Morphology in Human Tissues: Techniques and Applications presents the most promising and modern molecular morphological techniques to date. It explores a combination of morphology techniques, computer imaging, and 3D microscopy for the enhanced visualization and localization of peptides, proteins, DNA, and RNA at the genome level in various areas of biomedical research, clinical diagnostics, diagnostic molecular pathology, and histo- and cytogenetics. The pioneers of these specialized techniques contribute step-by-step protocols with tips, guidelines, and other suggestions from their own notebooks to achieve reproducibility, standardization, and economy. This format enables experienced scientists, as well as beginners, to apply the most progressive detection methods available and to obtain optimal results. This book offers extensive coverage of detection methods such as immunostaining techniques, the use of clustered gold, the detection of HER2 gene amplification, single gene molecular detection, the Southwest Histochemistry method, and interphase fluorescence.; Chapter 14 focuses on cost-efficient, 3D digital optical microscope technology, producing full color, 3D reproductions that allow researchers to analyze the spatial relations and abnormalities within cell structures in greater detail. The advanced, integrated techniques presented in Molecular Morphology in Human Tissues: Techniques and Applications provide powerful analytical and diagnostic tools used in a growing collaboration between research scientists and pathologists in various areas of biomedical research, clinical diagnostics, diagnostic molecular pathology, and histo- and cytogenetics. The application of these techniques will result in more cost-efficient, precise, and reliable diagnosis and management of cancer, viral infections, and other diseases.

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9781280289521

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