Overview

This text offers general information about molecular techniques relevant to molecular ecological research. Attention is paid to the use of molecular methods for studying the fate of genetically modified and native microorganisms in the environment.

Full Product Details

Author:   A. D. L. Akkermans ,  etc. ,  J.D. van Elsas (IPO-DLO, Wageningen, the Netherlands) ,  F.J. de Bruijn (MSU-DOE Plant Research Laboratory, Michigan State University, USA)
Publisher:   Kluwer Academic Publishers
Imprint:   Kluwer Academic Publishers
ISBN:  

9780792334101

ISBN 10:   0792334108
Pages:   488
Publication Date:   01 June 1995
Audience:   College/higher education ,  Professional and scholarly ,  Postgraduate, Research & Scholarly ,  Professional & Vocational
Format:   Loose-leaf
Publisher's Status:   Out of Print
Availability:   Out of stock  

Table of Contents

Extraction of Microbial DNA from Sewage and Manure; K. Smalla. Cell Extraction Method; V. Torsvik. Polymerase Chain Reaction (PCR) Analysis of Soil Microbial DNA; J. D. van Elsas, A. C. Wolters. Extraction and pcr Amplification of DNA from the Rhizoplane; P.A. Bramwell, R. V. Barallon, H. J. Rogers, M. J. Bailey. Extraction of Microbial DNA from the Phylloplane; P. A. Bramwell, R. V. Barallon, H. J. Rogers, M. J. Bailey. Extraction of Ribosomal DNA from Microbial Cultures; E. Stackebrandt, N. Ward. In situ Identification of Micro-organisms by Whole Cell Hybridization with RNA-Targeted Nucleic Acid Probes; R. I. Amann. Immunofluorescence Colony-Staining (IFC); J. W. L. van Vuurde, J. M. van der Wolf. Fluorescent Staining of Microbes for Total Direct Counts; J. Bloem. Natural Transformation in Aquatic Environments; J.H. Paul, H.G. Williams. Phage Ecology and Genetic Exchange in Soil; P.R. Herron. Design of Microcosms to Provide Data Reflecting Field Trials of GEMs; M.A. Hood, R. J. Seidler. Methods for Extracting DNA from Microbial Mats and Cultivated Micro-organisms: High Molecular Weight DNA from French Press Lysis; M.M. Bateson, D.M. Ward. Bacterial Community Fingerprinting of Amplified 16S and 16-23S Ribosomal DNA Gene Sequences and Restriction Endonuclease Analysis (ARDRA); A.A. Massol-Deya, D.A. Odelson, R.F. Hickey, J.M. Tiedje. Detection of Gene Transfer in the Environment: Conjugation in Soil; E. Smit, J.D. van Elsas. Direct and Simultaneous Extraction of DNA and RNA from Soil; S. Selenska-Pobell. Sequence Databases; W. Ludwig. Extraction of Microbial Community DNA from Soils; J.D. van Elsas, K. Malla. Investigation of Fungal Phylogeny on the Basis of Small Ribosomal Subunit RNA Sequences; Y. van de Peer, R. de Wachter. Biodegradation Genes as Marker Genes in Microbial Ecosystems; B.M. Applegate, U. Matrubutham, J. Sanseverino, G. S. Sayler. Preparation of Radioactive Probes; M. Cunningham. Detection of Nucleic Acids by Chemiluminescence; M. Cunningham, B. Harvey, M. Harris. Gel Purification of Soil DNA Extracts; D.D. Myrold, K.J. Martin, N.J. Ritchie. Detection of Microbial DNA Sequences by Colony Hybridization; P.R. Hirsch. Partial and Complete 16s RDNA Sequences, their Use in Generation of 16s RDNA Phylogenetic Trees and their Implications in Molecular Ecological Studies; E. Stackebrandt, F.A. Rainey. Extraction of Microbial DNA from Aquatic Sources: Freshwater; R.W. Pickup, G. Rhodes, J.R. Saunders. Slide Immunoenzymatic Assay (SIA); E. C. de Macario, A.J.L. Macario. Designing Release Experiments with GEMs in Foods; N. Klijn, A.H. Weerkamp, W. M. de Vos. Heavy Metal Resistances in Microbial Ecosystems; M. Mergeay. Detection of mRNA and rRNA via Reverse Transcription and PCR in Soil; S. Selenska-Pobell. Methods for Extracting RNA or Ribosomes from Microbial Mats and Cultivated Microorganisms; D.M. Ward, et al.

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