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Full Product Details

Author:   John R. Johnston (Reader in Microbial Genetics, University of Strathclyde)
Publisher:   Oxford University Press
Imprint:   Oxford University Press
Volume:   141
Dimensions:   Width: 14.50cm , Height: 1.70cm , Length: 23.40cm
Weight:   0.432kg
ISBN:  

9780199634293

ISBN 10:   0199634297
Pages:   300
Publication Date:   30 June 1994
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Out of Print
Availability:   In Print  
Limited stock is available. It will be ordered for you and shipped pending supplier's limited stock.

Table of Contents

1: Procedures for isolating yeast DNA for different purposes 2: Construction of cloning and expression vectors 3: Cosmid cloning of yeast DNA 4: The construction and use of cDNA libraries for genetic selections 5: Pulsed field gel electrophoresis 6: Plasmid shuffling and mutant isolation 7: Ty insertional mutagenesis 8: High efficiency transformation with lithium acetate 9: Measurement of transcription 10: Measurement of mRNA stability 11: Production of foreign proteins at high level 12: Cell-free translation of natural and synthetic mRNAs 13: Virus-like particles: Ty retrotransposons 14: The K1 killer toxin: molecular and genetic applications to secretion and cell surface assembly 15: Immuno-electron microscopy 16: Industrial Saccharomyces yeasts

Reviews

The distinguished collection of authors are often pioneers in their subject area and so the guidance is both authoritative and helpful. Overall this is a useful addition to the series, with ready access to standard lab protocols. Alan Wheals, Society for General Microbiology, November 1996

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