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Author:   María Isidoro García
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2016
Volume:   1434
Dimensions:   Width: 17.80cm , Height: 1.50cm , Length: 25.40cm
Weight:   5.525kg
ISBN:  

9781493981076

ISBN 10:   1493981072
Pages:   275
Publication Date:   30 May 2018
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Applications of Molecular Genetics to the Study of Asthma.- Assessment and Validation of New Genetic Variants: A systematic In Silico Approach.- Introduction to the Gene Expression Analysis.- Real Time PCR for Gene Expression Quantification in Asthma.- Validation of Reference Genes in mRNA Expression Analysis Applied to the Study of Asthma.- Review of Methods to Study Gene Expression Regulation Applied to Asthma.- Interactions of DNA and Proteins: Electrophoretic Mobility Shift Assay in Asthma.- SDS-Polyacrylamide Electrophoresis and Western Blotting Applied to the Study of Asthma.- Chromatin Immunoprecipitation: Application to the Study of Asthma.- Gene Silencing Delivery Methods: Lipid-Mediated and Electroporation Transfection Protocols.- Protocols for Exosome Isolation and RNA Profiling.- Cell Culture Techniques: Corticosteroid Treatment in A549 Human Lung Epithelial Cell.- Protocol for Lipid-Mediated Transient Transfection in A549 Epithelial Lung Cell Line.- Promoter Assay Using Luciferase Reporter Gene in the A549 Cell Line.- Review of Mouse Models Applied to the Study of Asthma.- General and Specific Mouse Models for Asthma Research.- Mouse Models applied to the Research of Pharmacological Treatments in Asthma.- Review on Pharmacogenetics and Pharmacogenomics Applied to the Study of Asthma.

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