Overview

The field of Molecular Endocrinology has exploded with the advent of new technological advances to study and understand the complex nature of endocrine interactions. This volume is designed to help investigators understand, appreciate, and be able to perform these techniques. It is a compilation of techniques from leading laboratories in the field. Each chapter provides a background of the technique and its applicability to the discipline of endocrinology. Chapters are designed to supply step by step technical aspects of the procedure including reagents and equipment needed along with discussions on troubleshooting and tips for avoiding procedural pitfalls. This book is targeted to investigators at all levels, from those early in their scientific careers to clinicians trying to gain an appreciation of how to perform new technologies to advanced scientists wishing to expand their repertoire of cutting edge techniques.

Full Product Details

Author:   Ok-Kyong Park-Sarge ,  Thomas E. Curry, Jr.
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Volume:   590
Dimensions:   Width: 19.30cm , Height: 2.80cm , Length: 26.00cm
Weight:   1.158kg
ISBN:  

9781603273770

ISBN 10:   1603273778
Pages:   430
Publication Date:   04 December 2009
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Out of stock  
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Table of Contents

Determining the Affinity of Hormone?Receptor Interaction.- Determination of Serum Estradiol Levels by Radiometric and Chemiluminescent Techniques.- Identification of Natural Human Glucocorticoid Receptor (hGR) Mutations or Polymorphisms and Their Functional Consequences at the Hormone–Receptor Interaction Level.- Monitoring Insulin-Stimulated Production of Signaling Lipids at the Plasma Membrane.- Gene Expression Profiling in the Aging Ovary.- Genomics Analysis: Endometrium.- Detection of Ovarian Matrix Metalloproteinase mRNAs by In Situ Hybridization.- Adenoviral Gene Transfer into Isolated Pancreatic Islets.- Basic Molecular Techniques for the Detection of Single Nucleotide Polymorphisms: Genome-Wide Applications in Search for Endocrine Tumor Related Genes.- Methylated DNA Immunoprecipitation and Microarray-Based Analysis: Detection of DNA Methylation in Breast Cancer Cell Lines.- Use of Reporter Genes to Study the Activity of Promoters in Ovarian Granulosa Cells.- Use of Reporter Genes to Study Promoters of the Androgen Receptor.- Isolation of Proteins Associated with the DNA-Bound Estrogen Receptor ?.- Chromosome-Wide Analysis of Protein Binding and Modifications.- Genome-Wide Analysis for Protein?DNA Interaction: ChIP-Chip.- Detection of ER?-SRC-1 Interactions Using Bioluminescent Resonance Energy Transfer.- Detection of Proteins Sumoylated In Vivo and In Vitro.- Identification of Alternative Transcripts Using Rapid Amplification of cDNA Ends (RACE).- Use of Laser Capture Microdissection in Studying Hormone-Dependent Diseases: Endometriosis.- Reporter Mice for the Study of Intracellular Receptor Activity.- Real-Time Non-invasive Imaging of ES Cell-Derived Insulin Producing Cells.- Transgenic Mouse Technology: Principles and Methods.- Breast Tumor-InitiatingCells Isolated from Patient Core Biopsies for Study of Hormone Action.- Markers of Oxidative Stress and Sperm Chromatin Integrity.- Planning and Executing a Genome Wide Association Study (GWAS).

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