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OverviewThis book gives a comprehensive account of the practical aspects of PCR and strong consideration is given to ensure its optimal use in a laboratory environment. This includes the theoretical aspects related to PCR, setting-up of a PCR laboratory and good Laboratory Practice and standardised PCR protocols to detect animal disease pathogens. Examples of Standard Operating Procedures as used in individual specialist laboratories and an outline of training materials necessary for PCR technology transfer are presented. The difficulties, advantages and disadvantages in PCR applications are explained and placed in context with other test systems. Emphasis is placed on the use of PCR for detection of pathogens with a particular focus on diagnosticians and scientists from the developing world. It is hoped that this book will enable readers from various disciplines and levels of expertise to better judge the merits of PCR and to increase their skills and knowledge in order to assist in a more logical, efficient and assured use of this technology. Full Product DetailsAuthor: Gerrit J. Viljoen , Louis H. Nel , John R. CrowtherPublisher: Springer Imprint: Springer Edition: Softcover reprint of hardcover 1st ed. 2005 Dimensions: Width: 16.00cm , Height: 1.70cm , Length: 24.00cm Weight: 0.526kg ISBN: 9789048168569ISBN 10: 9048168562 Pages: 308 Publication Date: 19 October 2010 Audience: Professional and scholarly , Professional & Vocational Format: Paperback Publisher's Status: Active Availability: Out of stock  The supplier is temporarily out of stock of this item. It will be ordered for you on backorder and shipped when it becomes available. Table of ContentsBackground.- PCR — The Basic Reaction.- Amplicon Detection, Analysis and PCR Evaluation.- Limitations and Troubleshooting.- Specific Types of PCR Assays.- The PCR Laboratory.- Disease Diagnosis Using PCR Specific Procedures for Important Veterinary Pathogens.ReviewsAuthor InformationTab Content 6Author Website:Countries AvailableAll regions |
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