Overview

Leading experts describe in step-by-step detail their most productive transposon-based methods and strategies for studying genome structure, function, and evolution. These readily reproducible techniques cover a wide range, including mutagenesis, transgenesis, gene silencing, and molecular systematics. Among the highlights are a series of DNA hybridization methods for analyzing the distribution and dynamics of mobile DNA at the hosts' genomic level, techniques for studying LTR retrotransposons in heterologous host systems, and mutagenesis protocols for investigating gene functions in a broad range of organisms. These cutting-edge methods offer investigators powerful genetic tools for dissecting the function of a specific gene, elaborating on the mechanisms leading to genetic change and diversity, and studying the evolutionary impact of mobile DNA on the biology and evolution of organisms.

Full Product Details

Author:   Wolfgang J. Miller ,  Pierre Capy
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. Softcover of orig. ed. 2004
Volume:   260
Dimensions:   Width: 15.20cm , Height: 1.70cm , Length: 22.90cm
Weight:   0.450kg
ISBN:  

9781617373015

ISBN 10:   161737301
Pages:   292
Publication Date:   10 November 2010
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Mobile Genetic Elements as Natural Tools for Genome Evolution.- Detection of Transposable Elements in Drosophila Salivary Gland Polytene Chromosomes by In Situ Hybridization.- Fluorescent In Situ Hybridization With Transposable Element Probes to Mitotic Chromosomal Heterochromatin of Drosophila.- Southern Blot Analysis of Individual Drosophila Flies.- Computational Analysis of Transposable Element Sequences.- Biochemical Analysis of Long Terminal Repeat Retrotransposons.- Tn5 as a Molecular Genetics Tool.- Gene Targeting in Drosophila.- TE-Based Mutagenesis Systems in Plants.- The Use of Double-Stranded RNA to Knock Down Specific Gene Activity.- The Application of LTR Retrotransposons as Molecular Markers in Plants.- MITE Display.- Retroposon Mapping in Molecular Systematics.- Transformation Systems in Insects.- Transposable Elements for Transgenesis and Insertional Mutagenesis in Vertebrates.

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