Overview

Full Product Details

Author:   Andrew D. McAinsh
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2009 ed.
Volume:   545
Dimensions:   Width: 19.30cm , Height: 2.50cm , Length: 26.00cm
Weight:   1.060kg
ISBN:  

9781603279925

ISBN 10:   160327992
Pages:   345
Publication Date:   29 June 2009
Audience:   College/higher education ,  Postgraduate, Research & Scholarly
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us.

Table of Contents

Knock-in and Knock-out: The Use of Reverse Genetics in Somatic Cells to Dissect Mitotic Pathways.- Functional Dissection of Mitotic Regulators Through Gene Targeting in Human Somatic Cells.- RNAi in Drosophila S2 Cells as a Tool for Studying Cell Cycle Progression.- Production of Mitotic Regulators Using an Autoselection System for Protein Expression in Budding Yeast.- Hydrodynamic Analysis of Human Kinetochore Complexes During Mitosis.- Isolation of Protein Complexes Involved in Mitosis and Cytokinesis from Drosophila Cultured Cells.- Automated Live Microscopy to Study Mitotic Gene Function in Fluorescent Reporter Cell Lines.- Electron Tomography of Microtubule End-Morphologies in C. elegans Embryos.- Dissecting Mitosis with Laser Microsurgery and RNAi in Drosophila Cells.- Fluorescence Imaging of the Centrosome Cycle in Mammalian Cells.- Visualization of Fluorescence-Tagged Proteins in Fission Yeast: The Analysis of Mitotic Spindle Dynamics Using GFP-Tubulin Under the Native Promoter.- Analysing Kinetochore Function in Human Cells: Spindle Checkpoint and Chromosome Congression.- Probing Kinetochore Structure and Function Using Xenopus laevis Frog Egg Extracts.- Live Cell Imaging of Kinetochore Capture by Microtubules in Budding Yeast.- The Spindle Checkpoint: Assays for the Analysis of Spindle Checkpoint Arrest and Recovery.- Measuring Proteolysis in Mitosis.- An In Vitro Assay for Cdc20-Dependent Mitotic Anaphase-Promoting Complex Activity from Budding Yeast.- In Vitro Assays for the Anaphase-Promoting Complex/Cyclosome (APC/C) in Xenopus Egg Extracts.- Preparation of Synchronized Human Cell Extracts to Study Ubiquitination and Degradation.- Biochemical Analysis of the Anaphase Promoting Complex: Activities of E2 Enzymes and Substrate Competitive (Pseudosubstrate) Inhibitors.

Reviews

From the reviews: McAinsh ! introduces mitosis as a phenomenally complicated process of the splitting of genetic material into two daughter cells that prevents genomic instability. Twenty chapters comprise this collection of laboratory protocols of up-to-date approaches and methods ! used to investigate the mechanism of mitosis at the molecular level. ! The volume is intended for graduate students and researchers in the field. (SciTech Book News, March, 2010)

From the reviews: McAinsh ... introduces mitosis as a phenomenally complicated process of the splitting of genetic material into two daughter cells that prevents genomic instability. Twenty chapters comprise this collection of laboratory protocols of up-to-date approaches and methods ... used to investigate the mechanism of mitosis at the molecular level. ... The volume is intended for graduate students and researchers in the field. (SciTech Book News, March, 2010)

From the reviews: McAinsh introduces mitosis as a phenomenally complicated process of the splitting of genetic material into two daughter cells that prevents genomic instability. Twenty chapters comprise this collection of laboratory protocols of up-to-date approaches and methods used to investigate the mechanism of mitosis at the molecular level. The volume is intended for graduate students and researchers in the field. (SciTech Book News, March, 2010)

Author Information

Tab Content 6

Author Website:  

Customer Reviews

Recent Reviews

No review item found!

Add your own review!

Countries Available

All regions