Overview

This fully updated edition explores the different pathways that converge into the regulation of mitochondrial function. The book integrates mitochondria with other cellular components, discussing the dynamic properties of mitochondria with an emphasis on how these processes respond to signaling events and how they affect cellular metabolism. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and up-to-date, Mitochondrial Regulation: Methods and Protocols, Second Edition is an ideal guide for advanced undergraduates, graduates, postgraduates, and beginning researchers in the areas of molecular and cellular biology, biochemistry, and bioenergetics.

Full Product Details

Author:   Carlos M. Palmeira ,  Anabela P. Rolo
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   2nd ed. 2021
Volume:   2310
Weight:   0.633kg
ISBN:  

9781071614358

ISBN 10:   1071614355
Pages:   318
Publication Date:   07 June 2021
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Isolation of Mitochondria from Liver and Extraction of Total RNA and Protein: Analyses of miRNA and Protein Expression.- Determination of Oxidative Phosphorylation Complexes Activities.- BN-PAGE-Based Approach to Study Thyroid Hormones and Mitochondrial Function.- Monitoring Mitochondrial Function in Mouse Embryonic Stem Cells (mESCs).- Mitochondrial Functional Assessment in Mammalian Gametes Using Fluorescent Probes.- Update on the Histoenzymatic Methods for Visualization of the Activity of Individual Mitochondrial Respiratory Chain Complexes in the Human Frozen Sections.- An Update on Isolation of Functional Mitochondria from Cell for Bioenergetics Studies.- PCR-Based Determination of Mitochondrial DNA Copy Number in Multiple Species.- Methods to Monitor Mitophagy and Mitochondrial Quality: Implications in Cancer, Neurodegeneration, and Cardiovascular Diseases.- Analysis of Pro-Apoptotic Protein Trafficking to and from Mitochondria.- Exploring Liver Mitochondrial Function by 13C-Stable Isotope Breath Tests: Implications in Clinical Biochemistry.- Protocols for Mitochondria as the Target of Pharmacological Therapy in the Context of Nonalcoholic Fatty Liver Disease (NAFLD).- The Use of Reactive Oxygen Species Production by Succinate-Driven Reverse Electron Flow as an Index of Complex 1 Activity in Isolated Brown Adipose Tissue Mitochondria.- Mitochondrial Regulation Assessment by 13C-NMR Isotopomer Analysis.- Assays for Determination of Cellular and Mitochondrial NAD+ and NADH Content.- Following the Dynamism of the Mitochondrial Network in T Cells.- Measuring PGC-1α and Its Acetylation Status in Mouse Primary Myotubes.

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