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Author:   Thomas J. Nicholls ,  Jay P. Uhler ,  Maria Falkenberg
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2023
Volume:   2615
Weight:   0.920kg
ISBN:  

9781071629246

ISBN 10:   1071629247
Pages:   469
Publication Date:   21 February 2024
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Isolation of functional mitochondria and pure mtDNA from murine tissues.- Isolating mitochondria, mitoplasts, and mtDNA from cultured mammalian cells.- Coupling differential centrifugation with exonuclease treatment and size exclusion       chromatography (DIFSEC) for purification of mtDNA from mammalian cells.- Isolation and Quality Control of Yeast Mitochondria.- Mitochondrial DNA isolation from plants.- Visualize the Distribution and Dynamics of Mitochondrial DNA (mtDNA) nucleoids with        Multiple Labelling Strategies.- Visualization of mtDNA using FISH.- In situ analysis of mitochondrial DNA synthesis using metabolic labeling coupled to     fluorescence microscopy.- Measurement of nucleoid size using STED microscopy.- How to quantify DNA compaction by TFAM with Acoustic Force Spectroscopy and Total          Internal Reflection Fluorescence microscopy.- Assessing TFAM binding to human mitochondrial DNA.- Identification of proximity interactors of mammalian nucleoid proteins by BioID.- Localization of mitochondrial nucleoids by transmission electron microscopy using the transgenic expression of the mitochondrial helicase TWINKLE and APEX2.- In vitro assays of TWINKLE function.- Rolling Circle Replication and Bypass of Damaged Nucleotides.- Studying mitochondrial nucleic acid synthesis utilizing intact isolated mitochondria.- Functional assessment of mitochondrial DNA maintenance by depletion and repopulation     using 2’,3’-dideoxycytidine in cultured cells.- Analysis of mitochondrial DNA replication by two-dimensional agarose gel electrophoresis.- Quantitative analysis of nucleoside triphosphate pools in mouse muscle using hydrophilic        interaction liquid chromatography coupled with tandem mass spectrometry detection.- Detection of UV-Induced Deletions in Mitochondrial DNA.- Determination of the ribonucleotide content of mtDNA using alkaline gels.- 5´-end mapping in human mitochondrial DNA.- Manipulation of murine mitochondrial DNA heteroplasmy with mtZFNs.- Biolistic transformation of Chlamydomonas reinhardtii and Saccharomyces cerevisiae      mitochondria.- A method for precisely identifying modifications to plant mitochondrial genomes by     mitoTALENs.- Mitochondrial DNA sequencing and heteroplasmy quantification by Next Generation     Sequencing.- Genomic strategies in mitochondrial diagnostics.- Mitochondrial DNA enrichment for sensitive next-generation sequencing.- Single cell analysis of mitochondrial DNA deletions.

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