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Author:   Anne Straube
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2011
Volume:   777
Dimensions:   Width: 17.80cm , Height: 1.80cm , Length: 25.40cm
Weight:   0.859kg
ISBN:  

9781493961856

ISBN 10:   1493961853
Pages:   319
Publication Date:   30 April 2017
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

How to Measure Microtubule Dynamics?.- Purification of Tubulin from Porcine Brain.- Purification of Tubulin from the Fission Yeast Schizosaccharomyces pombe.- Generation of Differentially Polyglutamylated Microtubules.- Preparation of Pure Tyrosinated or Detyrosinated Tubulin Isoforms.- ab-Tubulin and Microtubule Binding Assays.- Analysis of Microtubule Assembly Kinetics Using Turbidimetry.- Isolation of Centrosomes from Cultured Cells.- Preparation of Dual-Color Polarity-Marked Fluorescent Microtubule Seeds.- Reconstitution and Quantification of Dynamic Microtubule End Tracking In Vitro Using TIRF Microscopy.- Force Generation by Dynamic Microtubules In Vitro.- Variations on the Single Molecule Assay for Microtubule-Associated Proteins and Kinesins.- Analyzing the ATP Hydrolysis Cycle of Microtubule Motors.- Cryo-Electron Tomography of Microtubules Assembled In Vitro from Purified Components.- Kinetochore Microtubules, Analyzed by Correlated Light and Immuno-Electron Microscopy.- Photoactivatable-GFP-a-Tubulin as a Tool to Study Microtubule Plus-End Turnover in Living Human Cells.- Quantification of Asymmetric Microtubule Nucleation at Sub-Cellular Structures.- Investigating Microtubule Dynamic Instability Using Microtubule Targeting Agents.- L Isolation, Identification, and Validation of Microtubule-Associated Proteins from Drosophila Embryos.- Isolation of Novel +TIPs and Their Binding Partners Using Affinity Purification Techniques.

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