Overview

Histochemistry is a borderline field between histology and analytical chemistry or biochemistry. Its subject matter is the identification and localization of chemical substances in the tissues on a cytological scale. In the present book the term will be used in a more restricted sense to include only those methods in which the identifying chemical reaction is observed directly through the microscope, in tissues of which the architecture is not grossly altered. This definition will eliminate at once two other important ways of approach: (1) those in which certain morphological structures (nuclei, mitochondria, etc.) are first separated by physical means, such as differential solubility or centrifugation, and are then analyzed chemically and (2) the ingenious statistical methods developed by Linderstr0m-Lang DEGREES and his sc

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9781974347087

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