Overview

This updated volume reflects new and evolved techniques to study detection, profiling, and manipulation of microRNAs (miRNAs) in plants and animals. After overviews of how best to detect, identify, and validate microRNAs, the book continues by exploring state-of-the-art protocols for microRNA detection, approaches to profile the expression level of microRNAs, spatial expression analysis, describe in silico analysis of microRNAs and their targets, as well as protocols for functional analysis of microRNAs and their targets by CRISPR/Cas. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and up-to-date, MicroRNA Detection and Target Identification: Methods and Protocols, Second Edition aimsto ensure successful results in the further study of this vital field.

Full Product Details

Author:   Tamas Dalmay
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   2nd ed. 2023
Volume:   2630
Weight:   0.722kg
ISBN:  

9781071629819

ISBN 10:   1071629816
Pages:   266
Publication Date:   24 January 2023
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Detection of miRNAs             Afrah Bawazeer and David C. Prince   2. MicroRNA Identification, Target Prediction, and Validation for Crop Improvement             Vrantika Chaudhary, Sumit Jangra, Apurva Mishra, and Neelam R. Yadav   3. MicroRNA Detection with CRISPR/Cas             Xinyuan Qiu, Chuanyang Liu, Chushu Zhu, and Lingyun Zhu   4. Detection of MicroRNAs by Northern Blot             Claudia Martinho and Sara Lopez-Gomollon   5. MicroRNA Detection at Femtomolar Concentrations with Isothermal Amplification and a Biological Nanopore             Sotaro Takiguchi and Ryuji Kawano   6. Detection of MicroRNAs Expression Dynamics Using LNA/DNA Nanobiosensor             Yuwen Zhao and Shue Wang   7. Programmable Ultrasensitive Molecular Amplifier for Digital and Multiplex MicroRNA Quantification             Yannick Rondelez and Guillaume Gines   8. Small RNA Profiling by Next Generation Sequencing Using High-Definition Adapters             Rocky Payet and Martina Billmeier   9. Quantification of MicroRNAs or Viral RNAs with Microelectrode Sensors Enabled by Electrochemical Signal Amplification             Sarah Ake, Swagatika Kamila, and Gangli Wang   10. Discovery and Evaluation of Extracellular MicroRNA Biomarkers in Plasma, Ascites, and Urine             Luděk Záveský and Ondřej Slanař   11. Determining miRNA Expression Patterns in Xenopus             Marco Antonaci, Alice M. Godden, and Grant N. Wheeler   12. Overview of Computational and Experimental Methods to Identify Tissue-Specific MicroRNA Targets             Ulf Schmitz   13. sRNAtoolbox: Dockerized Analysis of Small RNA Sequencing Data in Model and Non-Model Species             Cristina Gómez-Martín, Ernesto Aparicio-Puerta, and Michael Hackenberg   14. MicroRNA-Target Identification: A Combinatorial In Silico Approach             Taufiqul K.M. Arif, Rachel K. Okolicsanyi, Larisa M. Haupt, and Lyn R. Griffiths   15. An Efficient CRISPR-Cas9 Method to Knockout miRNA Expression in Xenopus tropicalis             Alice M. Godden, Marco Antonaci, and Grant N. Wheeler   16. Interrogation of Functional miRNA-Target Interactions by CRISPR/Cas9 Genome Engineering             Qianxin Wu, Yale S. Michaels, and Tudor A. Fulga

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