Overview
As the number of sequenced genomes continues to increase, understanding the functions of newly discovered molecules will require greater efficiency and further study within the context of live cells. In Microinjection: Methods and Protocols, expert researchers contribute methods utilizing microinjection techniques ranging from expression of RNA to the integration of DNA into the genome with the ultimate goal of learning about gene expression, signal transduction, and protein function within these living cells. This versatile volume updates established techniques such as cRNA expression in Xenopus oocytes, and examines new, cutting-edge technologies, including antisense morpholino oligonucleotides, RNAi for knockdown experiments, and the use of integrase to produce transgenic animals, all through microinjection techniques that can be easily adopted by any lab. Written in the highly successful Methods in Molecular Biology series format, chapters include brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections, highlighting tips on troubleshooting and avoiding known pitfalls.Comprehensive and easily accessible, Microinjection: Methods and Protocols is an ideal source for new ideas and techniques certain to aid in the advance of biological research.
Full Product Details
Author: David J. Carroll
Publisher: Humana Press Inc.
Imprint: Humana Press Inc.
Edition: 2009 ed.
Volume: 518
Dimensions:
Width: 19.30cm
, Height: 1.50cm
, Length: 26.00cm
Weight: 0.687kg
ISBN: 9781588298843
ISBN 10: 1588298841
Pages: 214
Publication Date: 12 December 2008
Audience:
Professional and scholarly
,
Professional & Vocational
Format: Hardback
Publisher's Status: Active
Availability: In Print
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Reviews
From the reviews: Chapters cover all of the hot topics in which the technique can be used (namely, all the research field you are involved!) and so many different model systems ... associated with other master technique (e.g., western blot analysis). ... the beginners can immediately get good self-trainings tricks that will ensure them to reach good results. ... I would like all the readers ... to get a copy of the book: I am sure they will broad the paraphernalia usefull to carry on succesfull researches. (CarloAlberto Redi, European Journal of Histochemistry, October, 2009)
From the reviews: Chapters cover all of the hot topics in which the technique can be used (namely, all the research field you are involved!) and so many different model systems ... associated with other master technique (e.g., western blot analysis). ... the beginners can immediately get good self-trainings tricks that will ensure them to reach good results. ... I would like all the readers ... to get a copy of the book: I am sure they will broad the paraphernalia usefull to carry on succesfull researches. (CarloAlberto Redi, European Journal of Histochemistry, October, 2009)
From the reviews: Chapters cover all of the hot topics in which the technique can be used (namely, all the research field you are involved!) and so many different model systems ! associated with other master technique (e.g., western blot analysis). ! the beginners can immediately get good self-trainings tricks that will ensure them to reach good results. ! I would like all the readers ! to get a copy of the book: I am sure they will broad the paraphernalia usefull to carry on succesfull researches. (CarloAlberto Redi, European Journal of Histochemistry, October, 2009)
