Overview

This detailed book provides a set of protocols necessary for the development of a variety of microchip-based electrophoretic assays. It compiles a range of such electrophoretic methods by leading researchers in the field, covering subjects such as microfluidic device fabrication, on-chip sample preparation, theoretical/simulation protocols for assessing these separation methods, as well as common practices followed when applying them to important real world applications. The contents of the book range from protocols for classical assays to those involving pioneering separation techniques recently developed by the scientific community for advancing our analytical capabilities. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and accessible, Microfluidic Electrophoresis: Methods and Protocols serves as an convenient text for academic researchers as well as practicing engineers, biochemists, and analytical laboratory professionals.

Full Product Details

Author:   Debashis Dutta
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. 2019
Volume:   1906
Weight:   0.737kg
ISBN:  

9781493989638

ISBN 10:   1493989634
Pages:   255
Publication Date:   29 November 2018
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Fabrication of Glass Microfluidic Devices Christopher T. Culbertson, Jay Sibbitts, Kathleen Sellens, and Shu Jia 2. Soft Lithography, Molding, and Micromachining Techniques for Polymer Micro Devices Ashis Kumar Sen, Abhishek Raj, Utsab Banerjee, and Sk Rameez Iqbal 3. Sample Injection Techniques James M. Karlinsey 4. Sample Preconcentration Protocols in Microfluidic Electrophoresis Fumihiko Kitagawa and Koji Otsuka 5. Microchip Electrophoresis Containing Electrodes for Integrated Electrochemical Detection Lucas Paines Bressan, Dosil Pereira de Jesus, Dulan Bandara Gunasekara, Susan Marie Lunte, and Jose Alberto Fracassi da Silva 6. Micellar Electrokinetic Chromatography Braden C. Giordano, Ronald Seifert, and Greg E. Collins 7. Microchip Isotachophoresis: Analysis of Pharmaceuticals Marian Masar and Jasna Hradski 8. Microfluidic Free-Flow Isoelectric Focusing with Real-Time pI Determination Stefan Nagl 9. Nanochannel Gradient Separations Michael A. Startsev and David W. Inglis 10. Paper-Based Electrophoresis Microchip as a Powerful Tool for Bioanalytical Applications Cyro L.S. Chagas, Thiago M.G. Cardoso, and Wendell K.T. Coltro 11. Band Broadening Theories in Capillary Electrophoresis Sandip Ghosal 12. Estimating Stream Broadening in Free-Flow Electrophoretic Systems Based on the Method-of-Moments Formulation Debashis Dutta 13. Microchip Electrophoresis Tools for the Analysis of Small Molecules Federico J.V. Gomez and Maria Fernanda Silva 14. Integrated Microfluidic System for Rapid DNA Fingerprint Analysis: A Miniaturized Integrated DNA Analysis System (MiDAS): Swab Sample-In to DNA Profile-Out Jianing Yang, Cedric Hurth, Alan Nordquist, Stan Smith, and Frederic Zenhausern 15. Achieving Stable Electrospray Ionization Mass Spectrometry (ESI-MS) Detection from Microfluidic Chips Iulia M. Lazar 16. Microchip-Based Electrophoretic Separations with a Pressure-Driven Backflow Ling Xia and Debashis Dutta

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