Overview

Presently, the need for methods involving separation, identification, and characterization of different kinds of cells is amply realized among immu­ nologists, hematologists, cell biologists, clinical pathologists, and cancer researchers. Unless cells exhibiting different functions and stages of differ­ entiation are separated from one another, it will be exceedingly difficult to study some of the molecular mechanisms involved in cell recognition, spe­ cialization, interactions, cytotoxicity, and transformation. Clinical diagno­ sis of diseased states and use of isolated cells for therapeutic (e. g. , immu­ notherapy) or survival (e. g, transfusion) purposes are some of the pressing areas where immediate practical benefits can be obtained by applying cell separation techniques. However, the development of such useful methods is still in its infancy. A number of good techniques exist based either on the physical or biological properties of the cells, and these have produced some valuable results. Still others are to be discovered. Therefore, the purpose of this open-ended treatise is to acquaint the reader with some of the basic principles, instrumentation, and procedures presently in practice at various laboratories around the world and to present some typical applications of each technique to particular biological problems.

Full Product Details

Author:   Nicholas Catsimpoolas
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   Softcover reprint of the original 1st ed. 1980
Dimensions:   Width: 15.20cm , Height: 1.20cm , Length: 22.90cm
Weight:   0.328kg
ISBN:  

9781468436648

ISBN 10:   1468436643
Pages:   204
Publication Date:   18 March 2012
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1 Computer Analysis of Lymphocyte Images.- I. Introduction.- II. Selection of Equipment.- III. Computer Programs.- IV. Analytical Tasks.- V. Application of Image Analysis to Biological Problems.- References.- 2 Principles of Continuous Flow Cell Separation in a Circumferential Flow Disposable Channel.- I. Introduction.- II. Design Concepts.- III. Channel Description.- IV. Channel Operation.- V. Channel Performance.- VI. Summary.- References.- 3 Magnetic Microspheres in Cell Separation.- I. Background.- II. Procedures.- III. Examples.- References.- 4 Isolation of Human Blood Phagocytes by Counterflow Centrifugation Elutriation.- I. Introduction.- II. Isolation of Granulocytes from Human Blood.- III. Isolation of Human Blood.- IV. Appraisal of Counterflow Centrifugation for the Isolation of Leukocytes.- References.- 5 Biological Methods for the Separation of Lymphoid Cells.- I. General Considerations.- II. Rosetting Methods.- III. Affinity Chromatography of Cells and Cellular Immunoabsorbent Methods.- IV. Elimination of Lymphocyte Subpopulations by Antisera Plus Complement Treatment.- V. Elimination of Specific Lymphocyte Subpopulations by Selective Incorporation of High-Specific-Activity Radioisotopes (“Suicide” Methods).- VI. Separation of Lymphocyte Subpopulations by the Use of Lectins.- VII. Conclusions.- References.

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