Overview
Various methodologies designed to study cell walls are compiled in this book. Methods in Cell Wall Cytochemistry covers the use of modern dyes, fluorescent chemicals, lectins, and antibody technology (immunocytochemisty.) Cell wall morphology and chemical composition is covered as well as light and fluorescent cytochemistry; transmission electron microscopic cytochemistry; lectin cytochemistry; and, special emphasis on immunocytochemistry. Addressing an emerging area of research and technology, this book will appeal to plant pathologists, cell biologists, as well as workers interested in stress response and those employing cell walls for biotechnological research.
Full Product Details
Author: K V Krishnamurthy
Publisher: Taylor & Francis Ltd
Imprint: CRC Press
Weight: 0.453kg
ISBN: 9780367399863
ISBN 10: 0367399865
Pages: 336
Publication Date: 27 September 2019
Audience:
Professional and scholarly
,
Professional & Vocational
Format: Paperback
Publisher's Status: Active
Availability: In Print 
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Table of Contents
Preface Cell Wall-Morphology & Chemical Composition Carbohydrates Cellulose, Callose and other 1, 3-b-glucans, Chitins, Pectins, Hemicelluloses, Alginic Acid, Sulphated Polysaccharides Glycoproteins Structural Proteins-Lectins, Arabinogalacton Rich Proteins (AGP's), Hydroxy Proline Rich Proteins, Proline Rich Proteins, Glycine Rich Proteins, Threonine Rich Proteins, Thionins, Pistil Specific Extensin-Like Proteins Enzymes-Peroxidase, Polyamineoxidase, Phosphatase, Protease, Nitrate Reductase, Malate Dehydrogenase, Proline Hydroxylase, Glycosylhydrolases and Transferases, Non-Specific Esterases, and Ribonucleuses Lipids and Related Substances-Cutin, Suberin, Waxes, Sporopollenin, Pollenkitt, and Tryphine Lignin and Other Phenolic Substances-Lignins, Phenolic Acids and Amides Mineral Substances-Silica, Calcium, and Boron Light Microscopic Cytochemistry Introduction Tissue Preparation-Use of Fresh Living Tissue, Use of Frozen Tissue, Freeze Substitution and Drying, Chemical Fixation, Dehydration and Clearing, Infiltration and Embedding, Sectioning of Materials Stains and Staining-Introduction, Control Procedures, Incubation of Materials Mounting of Stained Materials Procedures in Tissue Preparation for Light Microscopic Cytochemistry-Freezing, Freeze Substitution and Drying Methods, Preparation of Fixatives, Dehydration and Clearing Procedures Procedures for the Cytochemical Localizations of Different Cell Wall Substances Carbohydrates-Insoluble Polysaccharides, Cellulose, Chitin, Callose, Sulphated and Carboxylated polysaccharides Proteins-Non-Specific Cell Wall Proteins, Enzymic Proteins Lignins Sporopollenins Cutins & Suberin Minerals Fluorescent Microscopic Cytochemistry Introduction Fluorescent Cytochemical Methods Cell Wall Carbohydrates Cell Wall Proteins Lignin Ferulic Acid Sporopollenin Cutin and Suberin Transmission Electron Microscopic Cytochemistry Introducti
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