Mesenchymal Stem Cells: Methods and Protocols

Author:   Massimiliano Gnecchi
Publisher:   Humana Press Inc.
Edition:   Softcover reprint of the original 2nd ed. 2016
Volume:   1416
ISBN:  

9781493980857


Pages:   570
Publication Date:   26 May 2018
Format:   Paperback
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

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Mesenchymal Stem Cells: Methods and Protocols


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Author:   Massimiliano Gnecchi
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 2nd ed. 2016
Volume:   1416
ISBN:  

9781493980857


ISBN 10:   1493980858
Pages:   570
Publication Date:   26 May 2018
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand   Availability explained
We will order this item for you from a manufactured on demand supplier.

Table of Contents

 Mesenchymal Stromal Cells In Hematopoietic Stem Cell Transplantation.-  Bone Tissue Engineering: Past – Present – Future.- Mesenchymal Stem Cells For Osteochondral Tissue Engineering.-  Mesenchymal Stem Cells In Cardiology.-  Mesenchymal Stem Cells In Kidney Repair.-  Mesenchymal Stem Cells In Lipogems, A Reverse Story: From Clinical Practice To Basic Science.- Paracrine Mechanisms Of Mesenchymal Stem Cells In Tissue Repair.-  Protocols For In Vitro Differentiation Of Human Mesenchymal Stem Cells Into Osteogenic, Chondrogenic And Adipogenic Lineages.- Colony Forming Unit Assays.-  Methods and Strategies For Lineage Tracing Of Mesenchymal Progenitor Cells.- Isolation Of Mouse Bone Marrow Mesenchymal Stem Cells.-  Isolation Of Pig Bone Marrow-Derived Mesenchymal Stem Cells.- Isolation, Culture, And Phenotypic Characterization Of Mesenchymal Stromal Cells From The Amniotic Membrane Of Human Term Placenta.-  Isolation, Culture And Characterization Of Human Umbilical Cord Blood-Derived Mesenchymal Stromal Cells.- Isolation, Expansion And Immortalization Of Human Adipose-Derived Mesenchymal Stromal Cells From Biopsies And Liposuction Specimens.-  Optimization Of Mesenchymal Stem Cells To Increase Their Therapeutic Potential.- Directed Differentiation Of Human Induced Pluripotent Stem Cells To Mesenchymal Stem Cells .- Isolation And Manufacture Of Clinical-Grade Bone Marrow-Derived Human Mesenchymal Stromal Cells.- Quality Control Assays For Clinical Grade Human Mesenchymal Stromal Cells: Methods For ATMP Release.-  Quality Control Assays For Clinical Grade Human Mesenchymal Stromal Cells: Validation Strategy.- Cryopreservation And Revival Of Human Mesenchymal Stromal Cells.-  Clinical-Grade Manufacturing Of Therapeutic Human Mesenchymal Stem/Stromal Cells in Microcarrier-Based Culture Systems.- GMP-Compliant Expansion of Clinical Grade Human Mesenchymal Stromal/Stem Cells Using a Closed Hollow-Fibre Bioreacto.- Engineering Small-Scale and Scaffold-Based Bone Organs Via Endochondral Ossification Using Adult Progenitor Cells.-  Fabrication Of Elasticity-Tunable Gelatinous Gel For Mesenchymal Stem Cell Culture.- Testing the Paracrine Properties of Human Mesenchymal Stem Cells Using Conditioned Medium.- Tips on How to Collect and Administer the Mesenchymal Stem Cell Secretome for Central Nervous System Applications.-   Soluble Factors from Human Fetal Bone Marrow-Derived Mesenchymal Stem Cells: Preparation of Conditioned Medium and its Effect On Tumor Cells.-  Isolation and Characterization of Exosome From Human Embryonic Stem Cell-Derived Cmyc-Immortalized Mesenchymal Stem Cells.- Transcriptomic Analysis of Adult Renal Derived Mesenchymal Stem-Like Cells.-  Proteomic Analysis of Mesenchymal Stem Cells.- Unraveling Mesenchymal Stem Cells’s Dynamic Secretome Through Non-Targeted Proteomics.- Identification Of Factors Produced And Secreted By Mesenchymal Stromal Cells With The SILAC Method.

Reviews

“‘Mesenchymal Stem Cells: Methods and Protocols’ represents an outstanding comprehensive work helping scientists to understand better the role of MSC and its secretome in regenerative medicine. … The book should not be missed in all biomedical research laboratories interested in the development of clinically compliant manufacturing of therapeutic MSC or their derivatives. The book provides a great help to PhD students and postdocs involved in the rapidly developing research field of personified medicine.” (Cestmir Altaner, Neoplasma, Vol. 63 (5), 2016)


`Mesenchymal Stem Cells: Methods and Protocols' represents an outstanding comprehensive work helping scientists to understand better the role of MSC and its secretome in regenerative medicine. ... The book should not be missed in all biomedical research laboratories interested in the development of clinically compliant manufacturing of therapeutic MSC or their derivatives. The book provides a great help to PhD students and postdocs involved in the rapidly developing research field of personified medicine. (Cestmir Altaner, Neoplasma, Vol. 63 (5), 2016)


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