Overview

'Stephen A.Baldwin has performed a service to the scientific community in compelling these technical approaches to membrane transport studies' Journal Cell ScienceMembrane Transport contains a collection of experimental protocols for the study of proteins responsible for the transport of small molecules across biological membranes. It includes information on the latest methods for production of transport proteins by recombinant DNA technology, and for their investigation by techniques accessible to any well-found laboratory.

Full Product Details

Author:   Stephen A. Baldwin (School of Biochemistry and Molecular Biology, University of Leeds)
Publisher:   Oxford University Press
Imprint:   Oxford University Press
Volume:   230
Dimensions:   Width: 18.90cm , Height: 1.90cm , Length: 24.60cm
Weight:   0.760kg
ISBN:  

9780199637041

ISBN 10:   0199637040
Pages:   342
Publication Date:   15 June 2000
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Out of Print
Availability:   Out of stock  

Table of Contents

Assay of membrane transport in cells and membrane vesicles Reconstitution of membrane proteins: the Ca2+-ATPase of sarcoplasmic reticulum The Xenopus oocyte expression system for the cDNA cloning and characterization of plasma membrane transport proteins Expression of foreign transport proteins in yeast Baculovirus-mediated overexpression of transport proteins The amplified expression, identification purification, assay and properties of hexahistidine-tagged bacterial membrane transport proteins Spectroscopic and kinetic approaches for probing the mechanisms of solute transporters Detection and analysis of glucose transporters using photolabelling techniques Computer prediction of transporter topology and structure Two-dimensional crystallization of membrane proteins

Reviews

The book is logically organized, contains many protocols, and should allow investigators to undertake work in this area with minimal problems. --Doody's<br> Some of us have been convinced for years that membrane proteins are, of course, the most important players in biology on this planet. Consequently, it has been painful to see the new initiatives on structural genomics flying ahead with global plans for high-resolution structures of thousands of cytosolic proteins in the next few years, while membrane proteins are simply dumped to one side as too difficult to handle in such a grand plan. The contents of this new volume in the Practical Approach series immediately attracted me, therefore, as a timely reminder that some devotees are still working hard to bring membrane proteins into the mainstream of structure-function analysis with all the promise of greater insight into mechanisms of transport function that this will surely generate. ... This volume ... should be a useful guide and stimulant to those laboratories now prepared to get stuck into the analysis of membrane proteins at the structural level. --Trends in Biochemical Sciences<br> The guide is well organized and easy to use. . .Researchers who are currently working in the area of membrane transport(or just thinking about it) will want to obtain a copy of Membrane Transport: a Practical Approach for their reference libraries --Biochemistry and Molecular Biology Education<br>

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