Overview

This detailed volume presents a series of methods exploring membrane trafficking research, ranging from genetics and high-resolution imaging to in vitro biochemical and biophysical assays. Covering virtually all the major trafficking branches, the book delves into the exocytic pathway, which focuses on cargo transport from the ER to the Golgi, through the Golgi cisternae, and to the plasma membrane and the extracellular space; the endocytic pathway, which includes cargo endocytosis, endosomal recycling, and lysosomal degradation; as well as emerging topics beyond the conventional exocytic and endocytic pathways. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Membrane Trafficking: Methods and Protocols provides techniques with broad applications as an ideal guide for junior researchers new to membrane trafficking as well as established membrane biologists seeking to expand their research programs.

Full Product Details

Author:   Jingshi Shen
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2022
Volume:   2473
Weight:   0.990kg
ISBN:  

9781071622087

ISBN 10:   1071622080
Pages:   407
Publication Date:   13 July 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I: The Exocytic Pathway   1. Characterizing Membrane Traffic in the Early Secretory Pathway Using the RUSH Retention System             Marine D. Camus and Stephane M. Camus   2. Monitoring Intra-Golgi Transport with Acute Spatiotemporal Control of a Synthetic Cargo             Julia Dancourt and Grégory Lavieu   3. Imaging Secretory Granule Budding from the Trans-Golgi Network Using Retention Using Selective Hook (RUSH)             Cedric S. Asensio   4. Spatial and Temporal Control of Protein Secretion with Light             Ashley M. Bourke and Matthew J. Kennedy   5. Generation of Endogenously Tagged Membrane Trafficking Regulators Using CRISPR Genome Editing             Syed Mukhtar Ahmed   6. Determine the Function of the Exocyst in Vesicle Tethering by Ectopic Targeting             Kunrong Mei, Di-Ao Liu, and Wei Guo   7. Imaging Insulin Granule Dynamics in Human Pancreatic β-Cells Using Total Internal Reflection Fluorescence (TIRF) Microscopy             Fei Kang and Herbert Y. Gaisano   8. Monitoring Synaptic Exocytosis Using a Whole-Cell Patch Clamp Technique             Jihong Gong, Xiaofei Yang, and Cong Ma   9. Live-Cell Super-Resolution Imaging of Retrograde Axonal Trafficking Using Pulse-Chase Labeling in Cultured Hippocampal Neurons             Tong Wang and Frédéric A. Meunier   10. Electron Tomographic Methods for Studying Organelles of the Murine Chemical Synapse             Tamara Basta, Garry P. Morgan, Eileen T. O'Toole, Nalini R. Rao, Jeffrey N. Savas, and Michael H.B. Stowell   11. In Vitro Reconstitution Studies of SNAREs and Their Regulators Mediating GLUT4 Vesicle Fusion             Yinghui Liu, Ruyue He, Min Zhu, and Haijia Yu   12. Imaging Single Vesicle Exocytosis with Total Internal Reflection Fluorescence Microscopy (TIRFM)             Yingke Xu, Luhong Jin, and Derek Toomre   Part II: The Endocytic Pathway   13. Localizing Proteins on Single Trafficking Organelles in 3D with Semisynthetic Gold Labeling and Platinum Replica Electron Microscopy             Bijeta Prasai and Justin W. Taraska   14. Endocytosis Assays Using Cleavable Fluorescent Dyes             Shifeng Wang, Chun Wan, Galen T. Squiers, and Jingshi Shen   15. Reconstituting and Purifying Assembly Intermediates of Clathrin Adaptors AP1 and AP2             Bing Wang, Rui Yang, Yuan Tian, and Qian Yin   16. Monitoring Endosomal Cargo Retrieval to the Trans-Golgi Network by Microscopic and Biochemical Approaches             Amulya Priya and Sunando Datta   17. Measuring Plasma Membrane Recycling Using Microscopic and Biochemical Approaches             Priyanka Sharma, Sameena Parveen, and Sunando Datta   18. Light Scattering Techniques to Assess Self-Assembly and Hydrodynamics of Membrane Trafficking Proteins             Marijn G.J. Ford and Rajesh Ramachandran   19. Measurement of Lysosome Positioning by Shell Analysis and Line Scan             Chad D. Williamson, Carlos M. Guardia, Raffaella De Pace, Juan S. Bonifacino, and Amra Saric   Part III: Emerging Topics in Membrane Trafficking   20. Optogenetic Control of Membrane Trafficking Using Light-Activated Reversible Inhibition by Assembly Trap of Intracellular Membranes (IM-LARIAT)             Bobae Hyeon, Mai Khanh Nguyen, and Won Do Heo   21. Generating Custom Pooled CRISPR Libraries for Genetic Dissection of Biological Pathways             Daniel R. Gulbranson   22. Studying Unconventional Secretion of Misfolded Proteins in Cultured Cells and Primary Neurons             Juhyung Lee and Yihong Ye   23. Single-Molecule Optical Tweezers Study of Protein-Membrane Interactions             Lu Ma, Jinghua Ge, Xin Bian, and Yongli Zhang   24. Generation of Hybrid Extracellular Vesicles by Fusion with Functionalized Liposomes             Max Piffoux, Amanda Karine Andriola Silva, Florence Gazeau, and David Tareste   25. Quantitative Measurement of Extracellular Vesicle Content Delivery within Acceptor Cells             Émeline Bonsergent, Shéryl Bui, and Grégory Lavieu

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