Overview

This extensive book brings together leading melanoma researchers from across the world and highlights many of the cutting-edge protocols and experimental systems currently being used to investigate questions surrounding this disease. The volume opens with sections on 2D and 3D cell culture-based approaches for studying melanoma biology, and continues with collections of chapters examining various approaches for detecting, isolating, and characterizing circulating melanoma cells, circulating tumor DNA, and exosomes, as well as experimental procedures for studying and detecting melanoma metastasis in both pre-clinical and clinical settings, bioinformatics-based approaches, protocols for quantifying and characterizing immune cell infiltrates in both melanoma tumors and tertiary lymphoid structures, and development and evaluation of therapeutic strategies for melanoma treatment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and comprehensive, Melanoma: Methods and Protocols aims to serve basic research scientists and clinicians who bring questions from the clinic into the lab in order to translate observations in the laboratory into improved patient care for this highly malignant form of cancer. Chapter 14 is available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Full Product Details

Author:   Kristian M. Hargadon
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2021
Volume:   2265
Weight:   1.303kg
ISBN:  

9781071612071

ISBN 10:   1071612077
Pages:   674
Publication Date:   13 March 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1. Model Systems for the Study of Malignant Melanoma             Randal K. Gregg   Part I: 2D Cell Culture-Based Approaches for Studying Melanoma Biology   2. Generation of Functional Gene Knockout Melanoma Cell Lines by CRISPR-Cas9 Gene Editing             Kristian M. Hargadon, David Z. Bushhouse, Coleman E. Johnson, and Corey J. Willams   3. A Fluorescent Gelatin Degradation Assay to Study Melanoma Breakdown of Extracellular Matrix             Ewa Mazurkiewicz, Ewa Mrówczyńska, Aleksandra Simiczyjew, Dorota Nowak, and Antonina J. Mazur   4. Wound Healing Assay for Melanoma Cell Migration             Juliano T. Freitas, Ivan Jozic, and Barbara Bedogni   5. A Fluorescence-Based Assay for Measuring Glucose Uptake in Living Melanoma Cells             Jelena Grahovac, Marijana Pavlović, and Marija Vidosavljević   6. Analyzing Melanoma Cell Oxygen Consumption and Extracellular Acidification Rates Using Seahorse Technology             Ashley V. Menk and Greg M. Delgoffe   7. Analysis of Melanoma Cell Glutamine Metabolism by Stable Isotope Tracing and Gas Chromatography-Mass Spectrometry             David A. Scott   8. Determination of Cytotoxic Activities Against Melanoma Cells Using Flow Cytometry             Guilan Shi and Richard Heller   9. In Vitro Differentiation of Tumor-Associated Macrophages from Monocyte Precursors with Modified Melanoma-Conditioned Medium             Tao Wang and Russel E. Kaufman   10. A Flow Cytometric Assay for Investigating Melanoma Cell Adhesion to Lymphatic Endothelial Cells             Kristian M. Hargadon and Coleman E. Johnson   Part II: 3D Cell Culture Systems for Studying Melanoma   11. An Approach to Study Melanoma Invasion and Crosstalk with Lymphatic Endothelial Cell Spheroids in 3D Using Immunofluorescence             Sanni Alve, Silvia Gramolelli, and Päivi M. Ojala   12. Evaluating Melanoma Viability and Proliferation in 3D Microenvironments             Vasanth Siruvallur Murali, Murat Can Cobanoglu, and Erik S. Welf   13. Preparation, Drug Treatment, and Immunohistological Analysis of Tri-Culture Spheroid 3D Melanoma-Like Models             Maximilian E.A. Schäfer, Julia Klicks, Mathias Hafner, and Rüdiger Rudolf   14. Enrichment of Melanoma Cancer Stem Cells via Sphere Assays             Nabanita Mukherjee, Karoline A. Lambert, David A. Norris, and Yiqun G. Shellman   Part III: Techniques for Isolating and Studying Circulating Melanoma Cells   15. Capture and Isolation of Circulating Melanoma Cells Using Photoacoustic Flowmetry             Robert H. Edgar, Justin Cook, Madeline Douglas, Anie-Pier Samson, and John A. Viator   16. Multi-Marker Immunomagnetic Enrichment of Circulating Melanoma Cells             Aaron B. Beasley, Emmanuel Acheampong, Weitao Lin, and Elin S. Gray   17. PD-L1 Detection on Circulating Melanoma Cells             Joseph W. Po, Yafeng Ma, Bavanthi Balakrishnar, Daniel Brungs, Farhad Azimi, Adam Cooper, Erin Saricilar, Vinay Murthy, Paul de Souza, and Therese M. Becker   18. Transcript-Based Detection of Circulating Melanoma Cells             Michael Morici, Weitao Lin, and Elin S. Gray   19. Isolation and Quantification of Plasma Circulating Tumor DNA from Melanoma Patients             Gabriela Marsavela, Anna Reid, Elin S. Gray, and Leslie Calapre   20. Simultaneous BRAFV600E Protein and DNA Aberration Detection in Circulating Melanoma Cells Using an Integrated Multi-Molecular Sensor             Alain Wuethrich, Shuvashis Dey, Kevin M. Koo, Abu A.I. Sina, and Matt Trau   21. Single Cell Analysis of BRAFV600E and NRASQ61R Mutation Status in Melanoma Cell Lines as Method Generation for Circulating Melanoma Cells             Joseph W. Po, Yafeng Ma, Alison W.S. Luk, David Lynch, Bavanthi Balakrishnar, Daniel Brungs, Farhad Azimi, Adam Cooper, Erin Saricilar, Vinay Murthy, Paul de Souza, and Therese M. Becker   Part IV: Methods to Study Melanoma-Derived and Melanoma-Associated Exosomes   22. A Rapid Exosome Isolation Using Size Exclusion Chromatography (REIUS) Method for Exosome Isolation from Melanoma Cell Lines             Shin La Shu, Cheryl L. Allen, Shawna Benjamin-Davalos, Marina Koroleva, Don MacFarland, Hans Minderman, and Marc S. Ernstoff   23. Immunoaffinity-Based Isolation of Melanoma Cell-Derived and T Cell-Derived Exosomes from Plasma of Melanoma Patients             Sujan Kumar Mondal and Theresa L. Whiteside   24. An Immunocapture-Based Assay for Detecting Multiple Antigens in Melanoma-Derived Extracellular Vesicles             Carmen Campos-Silva, Yaiza Cáceres-Martell, Sheila López-Cobo, María Josefa Rodriguez, Ricardo Jara, María Yáñez-Mó, and Mar Valés‐Gómez   25. Post-Lymphadenectomy Analysis of Exosomes from Lymphatic Exudate/Exudative Seroma of Melanoma Patients             Susana García-Silva, Pilar Ximénez-Embún, Javier Muñoz, and Héctor Peinado   Part V: Assessing Melanoma Metastasis in Pre-Clinical and Clinical Settings   26. Fate Mapping of Cancer Cells in Metastatic Lymph Nodes Using Photoconvertible Proteins             Ethel R. Pereira, Dmitriy Kedrin, and Timothy P. Padera   27. Detection of Melanoma Cells in Lymphatic Drainage (LD) after Lymph Nodes Dissection via Nested RT-PCR Analysis of Molecular Melanocytic Markers             Aleksandra Gos, Piotr Rutkowski, and Janusz A. Siedlecki   28. A Clonogenic Assay to Quantify Melanoma Micrometastases in Pulmonary Tissue             Fabrizio Mattei, Sara Andreone, and Giovanna Schiavoni   29. PET Imaging of Melanoma Using Melanin-Targeted Probe             Xiaowei Ma and Zhen Cheng   30. Imaging and Isolation of Extravasation-Participating Endothelial and Melanoma Cells During Angiopellosis             Tyler A. Allen and Ke Cheng   Part VI: Strategies for Diagnostic and Prognostic Biomarker Identification for Melanoma and Computational/Bioinformatic Approaches to OMICS Analysis of Melanoma   31. Salivary Proteomic Analysis of Canine Oral Melanoma by MALDI-TOF Mass Spectrometry and LC-Mass Spectrometry/Mass Spectrometry             Sekkarin Ploypetch, Sittiruk Roytrakul, and Gunnaporn Suriyaphol   32. Detection of Uveal Melanoma by Multiplex Immunoassays of Serum Biomarkers             Jin Song, Zhen Zhang, and Daniel W. Chan   33. Unbiased Microbiome and Metabolomic Profiling of Fecal Samples from Patients with Melanoma             Ashley Bui, Yongbin Choi, Arthur E. Frankel, and Andrew Y. Koh   34. Assessment of Cell-Free microRNA by NGS Whole Transcriptome Analysis in Cutaneous Melanoma Patients’ Blood             Kevin D. Tran, Rebecca Gross, Negin Rahimzadeh, Shanthy Chenathukattil, Dave S.B. Hoon, and Matias A. Bustos   35. High-Throughput Identification of miRNA-Target Interactions in Melanoma Using miR-CATCHv2.0             Andrea Marranci, Romina D’Aurizio, Milena Rizzo, Catherine M. Greene, and Laura Poliseno   Part VII: Characterization of Melanoma-Associated Tumor-Infiltrating Lymphocytes and Tertiary Lymphoid Structures   36. Immunotyping and Quantification of Melanoma Tumor–Infiltrating Lymphocytes             Max O. Meneveau, Zeyad T. Sahli, Kevin T. Lynch, Ileana S. Mauldin, and Craig L. Slingluff, Jr.   37. Single Cell Gene Expression, Clonality, and Feature Barcoding of Melanoma Tumor Infiltrating Lymphocytes             Angela Pizzolla, Simon Keam, Criselle D’Souza, Timothy Semple, and Paul J. Neeson   38. Using Mass Cytometry to Analyze the Tumor-Infiltrating Lymphocytes in Human Melanoma             Daniela Tantalo, Thu Nguyen, Han Xian Aw Yeang, Joe Zhu, Sean Macdonald, Minyu Wang, Harini de Silva, Criselle D’Souza, Angela Pizzolla, and Paul J. Neeson   39. Multiplex Immunohistochemistry Analysis of Melanoma Tumor Infiltrating Lymphocytes             Thu Nguyen, Nikolce Kocovski, Sean Macdonald, Han Xian Aw Yeang, Minyu Wang, and Paul J. Neeson   40. Multiplex Immunofluorescence Histology for Immune Cell Infiltrates in Melanoma-Associated Tertiary Lymphoid Structures             Ileana S. Mauldin, Adela Mahmutovic, Samuel J. Young, and Craig L. Slingluff, Jr.   Part VIII: Development and Evaluation of Therapeutic Strategies for Melanoma Treatment   41. The Use of Nanoparticles in the Delivery of Nucleic Acids for Melanoma Treatment             Mohammad A. Obeid, Alaa A.A. Aljabali, Meriem Rezigue, Haneen Amawi, Hanin Alyamani, Shatha N. Abdeljaber, and Valerie A. Ferro   42. siRNA Delivery to Melanoma Cells with Cationic Niosomes             Mohammad A. Obeid, Hanin Alyamani, Haneen Amawi, Alaa A.A. Aljabali, Meriem Rezigue, Shatha N. Abdeljaber, and Valerie A. Ferro   43. Controlled Delivery of Plasmid DNA to Melanoma Tumors by Gene Electrotransfer             Richard Heller and Guilan Shi   44. Generation of Murine Chimeric Antigen Receptor T Cells for Adoptive T Cell Therapy for Melanoma             Amorette Barber   45. Generation of Phosphopeptide-Specific T Cell Lines as Tools for Melanoma Immunotherapy             Rebecca C. Obeng and Angela L. Ambakhutwala

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