Overview

This volume details methods and protocols covering multiple aspects of Medulloblastoma. Divided into four parts, chapters guide readers through nucleic acids detection and analysis, cell-based analysis methodologies, and applications of patient-information on designing better experimental strategies for future drug development efforts in Medulloblastoma. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.   Authoritative and cutting-edge, Medulloblastoma: Methods and Protocols aims to deliver a clear-cut and standardized set of protocols to a broad scientific community.

Full Product Details

Author:   Abhinav Dey ,  Anshu Malhotra ,  Neha Garg
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2022
Volume:   2423
Weight:   0.485kg
ISBN:  

9781071619544

ISBN 10:   1071619543
Pages:   241
Publication Date:   05 January 2023
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

1.     Childhood Medulloblastoma: An Overview Yujin Suk, William D Gwynne, Ian Burns, Chitra Venugopal and Sheila K Singh   Part I: Molecular Assay-based Methods   2.     Real-time PCR assays on formalin-fixed paraffin embedded medulloblastomas Ratika Kunder and Neelam Vishwanath Shirsat 3.     Validation of microRNA target genes using luciferase reporter assay and western blot analysis Pooja Panwalkar, Atul Khire and Neelam Shirsat 4.     Chromatin Immunoprecipitation Assays on Medulloblastoma Cell Line DAOY Tara Dobson and Jyothishmathi Swaminathan 5.     Quantification of microRNAs in cells and tissues using stem-loop RT PCR and qPCR Pooja Panwalkar, Atul Khire and Neelam Shirsat 6.     Analysis of Microarray Data from Medulloblastoma tissue samples Debojyoti Dhar and Gopala Kallapura 7.     Advancement of single- cell sequencing in Medulloblastoma Deepanshu Verma, Namyashree Nayak, Ashuthosh Singh, Ashutosh Kumar Singh, Neha Garg   Part II: Cellular Assays and Inhibitors for Medulloblastoma 8.     Flow cytometry approaches to obtain medulloblastoma stem cells from bulk cultures Praveen Kumar and Neha Garg 9.     Drug Screening assays on Medulloblastoma Stem Cells using compound libraries Ashutosh Singh and Neha Garg 10.  3D-on-2D: A physiologically-relevant and gel-free in vitro co-culture method to assay anti-metastatic agents Abhinav Dey, Samuel Eisenberg, Rayna Birnbaum and David J Sharp 11.  In vitro screening assay for activators of T- cell migration to solid tumors Abhinav Dey and David Sharp 12.  Impact of Nano preparation of phytoconstituents in Medulloblastoma Priyanka Mishra and Y.B. Tripathi 13.  Challenges and Recent Advances of novel chemical inhibitors in Medulloblastoma Therapy Anand Maurya, Upendra Kumar Patel, Jitendra Kumar Yadav, Virender Pratap Singh, Alka Agarwal 14.  Role of ayurvedic plants as anti-cancer agents Nikhil Pandey and Y.B. Tripathi   Part III: Animal Models in Medulloblastoma 15.  In vivo tumorigenicity assays using subcutaneous and orthotopic xenografts in immunodeficient mice Kedar Yogi, Rahul Thorat and Neelam Shirsat 16.  Manufacture of an Oncolytic Measles Virus-Sensitive Immune-Competent Mouse Model of Medulloblastoma. Sangeet Lal and Corey Raffel   Part IV: Patient data based approaches for Biomedical Research in Medulloblastoma 17.  Preclinical Research Strategy Development for RNAi-based therapies in Oncology using patient-centred information from public databases of Human Protein Atlas and R2Genomics Platform Abhinav Dey, Isabelle Balachandran, Ava Solis and David J Sharp 18.  Magnetic resonance imaging in the contemporary management of medulloblastoma: current and emerging applications  Archya Dasgupta, Madan Maitre, Sona Pungavkar and Tejpal Gupta 19.  Multidisciplinary Management of Medulloblastoma:  Consensus, Challenges and Controversies Abhishek Chatterjee, Madan Maitre, Archya Dasgupta, Epari Sridhar and Tejpal Gupta

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