Overview

This detailed book collects methodologies exploring mechanobiology, the involvement of mechanical forces in cell fate specification and in controlling single and collective cell behaviors such as directed migration, morphogenesis, wound healing, and the immune response. The volume features methods to quantify the mechanical properties of cells and adhesion proteins, to expose cells to external mechanical forces, to quantitatively characterize mechano-responses at various scales, to measure forces applied by cells on the extracellular matrix, as well as chapters on force measurement inside cells, probing cell signaling and gene expression in response to force, and biophysical modeling of cell shape and protein dynamics. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary material and reagents, step-by-step and readily reproducible protocols, and tips on troubleshooting and avoiding known pitfalls.  Authoritative and practical, Mechanobiology: Methods and Protocols aims to provide meaningful tools for cell and developmental biologists approaching the study of cell and tissue dynamics from a mechanobiological perspective, molecular biologists interested in the effects of force on proteins, as well as for cancer biologists and biophysicists.

Full Product Details

Author:   Ronen Zaidel-Bar
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2023
Volume:   2600
Weight:   0.867kg
ISBN:  

9781071628508

ISBN 10:   107162850
Pages:   343
Publication Date:   02 January 2023
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I: Quantifying the Mechanical Properties of Cells and Adhesion Proteins   1. Measuring Cell Mechanical Properties Using Microindentation             Julien Husson   2. In Situ Measurements of Cell Mechanical Properties Using Force-Spectroscopy             Meital Reches   3. Quantification of Apparent Membrane Tension and Membrane-to-Cortex Attachment in Animal Cells Using Atomic Force Microscopy-Based Force Spectroscopy             Martin Bergert and Alba Diz-Muñoz   4. Characterizing the Biophysical Properties of Adhesive Proteins in Live Cells Using Single Molecule Atomic Force Microscopy             Andrew Vae Priest and Sanjeevi Sivasankar   Part II: Exposing Cells to External Mechanical Forces   5. Application of Shear Stress to Endothelial Cells Using a Parallel Plate Flow Chamber             Jason Sedlak and Alisa Clyne   6. Cell Stretcher Assay to Analyze Mechanoresponses to Cyclic Stretching             Robin Püllen, Jens Konrad, Bernd Hoffmann, and Rudolf Merkel   7. Two-Point Optical Manipulation of Cell Junctions in the Early Epithelium of the Drosophila Embryo             Kenji Nishizawa, Claire Chardès, Raphaël Clément and Pierre-François Lenne   Part III: Quantitative Characterization of Cell Mechanosensing   8. A Microfluidic-Like System (MLS) to Grow, Image, and Quantitatively Characterize Rigidity-Sensing by Plant’s Roots and Root Hair Cells             David Pereira, Thomas Alline, Gaurav Singh, Marie-Edith Chabouté, and Atef Asnacios   9. Photoresponsive Hydrogels for Studying Mechanotransduction of Cells             Keun-Young Park, David J. Odde, and Mark D. Distefano   10. Analyzing Mechanically Evoked Currents at Cell-Substrate Junctions             Surabhi Shrestha, Jessica Richardson, and Kate Poole   11. Quantifying Strain Sensing Protein Recruitment During Stress Fiber Repair             Shailaja Seetharaman, Stefano Sala, Margaret L. Gardel, and Patrick W. Oakes   Part IV: Measuring Forces Applied by Cells on the Extracellular Matrix   12. Quantification of Invadopodia Formation and Matrix Degradation Activity             Jubina Balan Venghateri and Benjamin Geiger   13. Measuring Cellular Traction Forces with Micropillar Arrays             Malak Amer and Haguy Wolfenson   14. Imaging Cell Adhesive Force at the Single Molecule Level             Ying Tu and Xuefeng Wang   Part V: Force Measurement Inside Cells   15. Multiplexed Molecular Tension Sensor Measurements Using PIE-FLIM             Lukas Windgasse and Carsten Grashoff   16. Visualizing Neurons under Tension In Vivo with Optogenetic Molecular Force Sensors             Neus Sanfeliu-Cerdán, Li-Chun Lin, Alexander R. Dunn, Miriam B. Goodman, and Michael Krieg   17. Single-Cell Quantification of the Mechanical Stability of Cell-Cell Adherens Junction Using Glass Micropipettes             Wenmao Huang, Chaoyu Fu, and Jie Yan   Part VI: Probing Cell Signaling and Gene Expression in Response to Force   18. Using Micropatterned Supported Lipid Bilayers to Probe the Mechanosensitivity of Signaling Receptors             Zhongwen Chen   19. Monitoring Mechano-Regulation of Gene Expression by RNA Sequencing             Yekaterina A. Miroshnikova   20. Testing the Role of Focal Adhesion Kinase (FAK) in Topography-Mediated Stem Cell Differentiation by Inhibiting FAK Phosphorylation             Yuan Yao and Evelyn K.F. Yim   Part VII: Biophysical Modeling of Cell Shape and Protein Dynamics   21. Long-Term Fluorescence Recovery After Photobleaching (FRAP)             Takumi Saito, Daiki Matsunaga, and Shinji Deguchi   22. Simulating 3D Cell Shape with the Cellular Potts Model             Rabea Link and Ulrich S. Schwarz

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