Overview

The study of signal transduction mechanisms has become one of the most important branches of biomedical science, indispensable to understanding the normal actions of hormones, neurotransmitters and other extracellular signaling molecules as well as many pathophysiological processes, including inflammation and cancer. In this volume, the various techniques for measuring Ca2+ in the cytosol, in the various organelles, and in the immediate surroundings of individual cells are described in practical detail by experts. Also included is a chapter on tracking calmodulin inside cells using fluorescence technology. A Springer Lab Manual

Full Product Details

Author:   Ole H. Petersen
Publisher:   Springer-Verlag Berlin and Heidelberg GmbH & Co. KG
Imprint:   Springer-Verlag Berlin and Heidelberg GmbH & Co. K
Edition:   2001 ed.
Dimensions:   Width: 15.50cm , Height: 1.70cm , Length: 23.50cm
Weight:   0.480kg
ISBN:  

9783642477379

ISBN 10:   3642477372
Pages:   301
Publication Date:   15 April 2014
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Cytosolic Ca2+ Concentration Measurements.- Principles of Fluorescence Measurements — Dyes and Hardware Required.- Simple Principles of Imaging — Image Files, Image Processing and Image Analysis.- Aequorin Measurements of Cytosolic Ca2+.- Electrophysiological Measurements Using Ca2+-Sensitive Microelectrodes.- Measuring Ca2+ Inside Organelles.- Measuring Ca2+ in the Nucleoplasm of Intact Cells.- Measuring Ca2+ in Isolated Nuclei.- Ca2+ Measurement with Luminescent Probes in the Endoplasmic Reticulum.- Ca2+ Measurement with Fluorescent Probes in the Endoplasmic Reticulum.- Ca2+ Measurements in Mitochondria.- Ca2+ Measurements in Secretory Granules.- Ca2+ Measurement in Endosomes of Intact Cells.- Misellaneous.- Measuring Ca2+ Extrusion from Single Cells.- Tracking Calmodulin Inside Cells.

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