Overview
This detailed volume provides an overview of recent advances in the application of genomic technologies in several domains of marine biology, raising awareness of various DNA- and RNA-based technologies. Genomic methods are essential in identifying previously undetected taxonomic (e.g. DNA barcoding), genetic (e.g. sequencing), and functional (e.g. gene expression, analysis of metabolites) diversity, as shown in the chapters of this book, with sections focusing on next generation sequencing (NGS) technologies, bioinformatics in marine genomics research, marine biotechnology, as well as a variety of methods successfully applied in fish. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Marine Genomics: Methods and Protocols highlights the utility of numerous lab protocols and their potential to provide deeper insight into physiological and ecological mechanisms in marine life.
Full Product Details
Author: Cinzia Verde , Daniela Giordano
Publisher: Springer-Verlag New York Inc.
Imprint: Springer-Verlag New York Inc.
Edition: 1st ed. 2022
Volume: 2498
Weight: 1.027kg
ISBN: 9781071623121
ISBN 10: 1071623125
Pages: 426
Publication Date: 22 June 2022
Audience:
Professional and scholarly
,
Professional & Vocational
Format: Hardback
Publisher's Status: Active
Availability: Manufactured on demand 
We will order this item for you from a manufactured on demand supplier.
Table of Contents
1. Mitochondrial Genome of Non-Model Marine Metazoans by Next-Generation Sequencing (NGS) Tullia I. Terraneo, Kiruthiga G. Mariappan, Zac Forsman, and Roberto Arrigoni 2. Genome-Wide DNA Methylation Protocol for Epigenetics Studies Annalaura Mancia 3. Transcriptome Mining to Identify Genes of Interest: From Local Databases to Phylogenetic Inference Daniele De Luca and Chiara Lauritano 4. Detecting Structural Variants and Associated Gene Presence-Absence Variation Phenomena in the Genomes of Marine Organisms Marco Sollitto, Nathan J. Kenny, Samuele Greco, Carmen Federica Tucci, Andrew D. Calcino, and Marco Gerdol 5. From Sequences to Enzymes: Comparative Genomics to Study Evolutionary Conserved Protein Functions in Marine Microbes Angelina Cordone, Alessandro Coppola, Angelica Severino, Monica Correggia, Matteo Selci, Antonio Cascone, Costantino Vetriani, and Donato Giovannelli 6. VenomFlow: An Automated Bioinformatic Pipeline for Identification of Disulfide-Rich Peptides from Venom Arsenals Eleonora Achrak, Jennifer Ferd, Jessica Schulman, Trami Dang, Konstantinos Krampis, and Mande Holford 7. Population Genomics Analysis with RAD, Reprised: Stacks 2 Angel G. Rivera-Colón and Julian Catchen 8. A Metabarcoding Protocol to Analyze Coastal Planktic Communities Collected by Desalination Plant Filters: From Sampling to Bioinformatic Exploratory Analyses Matteo Cecchetto, Andrea Di Cesare, Ester Eckert, Isabella Moro, Diego Fontaneto, and Stefano Schiaparelli 9. Barcoding of Antarctic Marine Invertebrates: From Field Sampling to Lab Procedures Stefano Schiaparelli, Maria Chiara Alvaro, Matteo Cecchetto, and Alice Guzzi 10. DNA Barcoding Procedures for Taxonomical and Phylogenetic Studies in Marine Animals: Porifera as Case Study Laura Núñez-Pons, Valerio Mazzella, Francesca Rispo, Jana Efremova, and Barbara Calcinai 11. Environmental DNA from Marine Waters and Substrates: Protocols for Sampling and eDNA Extraction Dominique A. Cowart, Katherine R. Murphy, and C.-H. Christina Cheng 12. Metataxonomic Analysis of Bacterial Diversity Associated with Marine Organisms Nadia Ruocco, Roberta Esposito, Valerio Zupo, and Maria Costantini 13. From Sequences to Enzymes: Heterologous Expression of Genes from Marine Microbes Angelica Severino, Alessandro Coppola, Monica Correggia, Costantino Vetriani, Donato Giovannelli, and Angelina Cordone 14. Expression of Recombinant Cold-Adapted (Hemo)Globins from Marine Bacteria Daniela Giordano and Cinzia Verde 15. Isolation of UV-Resistant Marine Bacteria by UV-C Assays Daniela Coppola, Cinzia Verde, and Daniela Giordano 16. Fractionation Protocol of Marine Metabolites Genoveffa Nuzzo, Emiliano Manzo, Carmela Gallo, Giuliana d'Ippolito, and Angelo Fontana 17. Detection and Quantification of Small Non-Coding RNAs in Marine Diatoms Alessandra Rogato and Angela Falciatore 18. Optimized Proteolistic Protocol for the Delivery of the Cas9 Protein in Phaeodactylum tricornutum Monia Teresa Russo, Anna Santin, Alessandra Rogato, and Maria Immacolata Ferrante 19. Production of a Chimeric Mouse-Fish Monoclonal Antibody by the CRISPR/Cas9 Technology Alessia Ametrano and Maria Rosaria Coscia 20. Identification, Characterization, and Expression Analysis of Immunoglobulin Genes from Antarctic Fish by PCR Methods Alessia Ametrano and Maria Rosaria Coscia 21. Physical Mapping of Repeated Sequences on Fish Chromosomes by Fluorescence In Situ Hybridization (FISH) Laura Ghigliotti, Juliette Auvinet, and Eva Pisano 22. Functional Genomics of Fish Erythrocytes Mikko Nikinmaa and Amélie Crespel 23. Stain-Free Approach for Western Blot Analysis of Zebrafish Embryos Jianmin Xue and Paola Corti 24. Proteomics of Fish White Muscle and Western Blotting to Detect Putative Allergens Liliana Anjos, Arsenios-Zafeirios Loukissas, and Deborah Mary Power 25. In Vitro Assays for the Bifunctional Acylpeptide Hydrolase (APEH) Enzyme from Antarctic Fish Carmela Fusco, Bruna Agrillo, Marta Gogliettino, Gianna Palmieri, and Ennio Cocca
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