Overview

This volume details methods of identifying synthetic lethal, genetic interactions by various approaches in different model systems including human cancer cells. Chapters guide readers through genetic interactions in model organisms, RNA interference, CRISPR/Cas9 based genome editing technologies, drug-gene interactions, mapping chemical genetic interactions, synergistic drug-gene relations, single cell sequencing, gene expression profiling, and novel genetic interactions. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.   Authoritative and cutting-edge, Genetic Interaction Mapping aims to be a useful practical guide to researches to help further their study in this field.

Full Product Details

Author:   Franco Joseph Vizeacoumar ,  Andrew Freywald
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2021
Volume:   2381
Weight:   0.721kg
ISBN:  

9781071617427

ISBN 10:   1071617427
Pages:   361
Publication Date:   01 October 2022
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I: Mapping Genetic Interactions in Model Systems 1. Quantitative Genetic Screens for Mapping Bacterial Pathways and Functional Networks Alla Gagarinova, Ali Hosseinnia, and Mohan Babu   2. Mapping Synthetic Dosage Lethal Genetic Interactions in Saccharomyces cerevisiae Akil Hamza, Leanne Amitzi, Supipi Duffy, and Philip Hieter   3. Systematic High-Content Screening of Fluorescently-Tagged Yeast Double Mutant Strains Harsha Garadi Suresh and Mojca Mattiazzi Usaj   4. A Genetic Interaction Screening Approach in C. elegans John A. Calarco   5. RNA Interference (RNAi) Screening in Cultured Drosophila Cells Juan-Carlos A. Padilla, Ashley Chin, Dhara Patel, Xiaofeng Wang, Philippe Jolivet, and Eric Lécuyer     Part II: Approaches to Map Genetic Interactions in Human cells 6. Employing Cross-Species Approaches to Construct Humanized Genetic Interaction Networks Lucile M. Jeusset and Kirk J. McManus   7. Identification of Synthetic Lethal Interactions Using High-Throughput, Arrayed CRISPR/Cas9-based Platforms Mackenzie J. MacAuley, Omar Abuhussein, and Frederick S. Vizeacoumar   8. Exploring Candidate Human Synthetic Lethal Interactions Through siRNA and Quantitative Imaging-Based Approaches Lucile M. Jeusset and Kirk J. McManus   9. Mapping Genetic Interactions in Human Cancer Cells Using a One-Step tRNA-CRISPR System Derek Yisen Zhang, Xiong Gui, and Xiaolong Yang   10. In vivo Genome-Wide Pooled RNAi Screens in Cancer Cells to Identify Determinants of Chemotherapy/Drug Response Margaret L Dahn and Paola Marcato   Part III: Predicting Genetic Interactions Using Computational Approaches 11. INCISOR: An Algorithm to Identify Synthetic Rescue Mediators of Resistance to Targeted and Immunotherapy Xiaoman Wang, Frederick Sagayaraj Vizeacoumar, and Avinash Das Sahu   12. Machine Learning to Identify Gene Interactions from High-Throughput Mutant Crosses Ashwani Kumar, Andrew Cameron, and Sandra Zilles   13. Identification of Drug Resistance Genes Using a Pooled Lentiviral CRISPR/Cas9 Screening Approach Evan M. Kerek, Christopher R. Cromwell, and Basil P. Hubbard   14. Chemical Genetic Interactions as a Means to Characterize Drug Synergy Hamid Gaikani, Guri Giaever, and Corey Nislow     Part V: Emerging Technologies that Leverage Mapping Genetic Interactions 15. Puromycin Labeling Coupled with Proximity Ligation Assays to Define Sites of mRNA Translation in Drosophila Embryos and Human Cells Ashley Chin and Eric Lécuyer   16. Inferring Copy Number from Triple Negative Breast Cancer Patient Derived Xenograft scRNAseq Data using scCNAutils Elena Kuzmin, Jean Monlong, Constanza Martinez Ramirez, Hellen Kuasne, Claudia L. Kleinman, Jiannis Ragoussis, Guillaume Bourque, and Morag Park   Part VI: Downstream Validation of Genetic Interactions using Protein Inhibitors and Small Molecules   17. Generation of Protein Inhibitors for Validation of Cancer Drug Targets Identified in Functional Genomic Screens Sherin McDonald, Arunkumar Annan Sudarsan, Hanan Babeker, Kiranmayee Budharaju, and Maruti Uppalapati   18. Computational Prediction of Chemical Tools for Identification and Validation of Synthetic Lethal Interaction Networks Kalpana K Bhanumathy, Omar Abuhussein, Frederick S. Vizeacoumar, Andrew Freywald, Franco J. Vizeacoumar, Christopher P. Phenix, Eric W. Price, and Ran Cao

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