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Author:   Martin J. Stoddart
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   2011
Volume:   740
Dimensions:   Width: 17.80cm , Height: 1.80cm , Length: 25.40cm
Weight:   0.678kg
ISBN:  

9781617791079

ISBN 10:   1617791075
Pages:   240
Publication Date:   12 April 2011
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print  
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Table of Contents

Cell Viability Assays: Introduction.- Cell Viability Analysis Using Trypan Blue: Manual and Automated Methods.- Estimation of Cell Number Based on Metabolic Activity: The MTT Reduction Assay.- WST8 Analysis of Cell Viability During Osteogenesis of Human Mesenchymal Stem Cells.- Assessment of Cell Proliferation with Resazurin-Based Fluorescent Dye.- The xCELLigence System for Real-Time and Label-Free Monitoring of Cell Viability.- Analysis of Tumor and Endothelial Cell Viability and Survival Using Sulforhodamine B (SRB) and Clonogenic Assays.- Annexin V / 7-AAD Staining in Keratinocytes.- Measurement of Caspase Activity: From Cell Populations to Individual Cells.- Rapid Quantification of Cell Viability and Apoptosis in B-Cell Lymphoma Cultures Using Cyanine SYTO Probes.- Multiplexing Cell Viability Assays.- Cytotoxicity Testing: Measuring Viable Cells, Dead Cells, and Detecting Mechanism of Cell Death.- Fluorescein Diacetate for Determination of Cell Viability in 3D Fibroblast-Collagen-GAG Constructs.- Confocal Imaging Protocols for Live/Dead Staining in 3-Dimensional Carriers.- Viability Assessment of Osteocytes Using Histological Lactate Dehydrogenase Activity Staining on Human Cancellous Bone Sections.- Measuring Glutamate Receptor Activation-Induced Apoptotic Cell Death in Ischemic Rat Retina Using the TUNEL Assay.- Exploiting the Liberation of Zn2+ to Measure Cell Viability.- Non-Invasive Bioluminescent Quantification of Viable Stem Cells in Engineered Constructs.- Raman Micro-Spectroscopy as a Non-Invasive Cell Viability Test.- Closed Ampoule Isothermal Microcalorimetry (IMC) for Continuous Real-Time Detection and Evaluation of Cultured Mammalian Cell Activity and Responses.-Digital Image Processing of Live/Dead Staining.

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