Overview

Antibodies are an indispensable tool in the study of biology and medicine. Making and Using Antibodies: A Practical Handbook presents techniques in a single, comprehensive source for the production and use of antibodies. It enables researchers to immediately access lab-tested, proven protocols. Written and edited by an elite team of scientists, who have developed and refined many of the methods, this book covers- Commercially available adjuvants designed for the production of antisera in the research setting Methods for the production, purification, and characterization of antibodies Practical guidance to researchers needing to modify antibodies Basic techniques including enzyme-linked immunosorbent assay (ELISA), Western blotting, immunohistochemistry, and flow cytometry Methods for applying immunolabeling to the realm of electron microscopy (EM) New antibody sources and new ways to use antobodies Making and Using Antibodies: A Practical Handbook also offers invaluable insight into future directions, challenges, and opportunities both in research and industrial applications.

Full Product Details

Author:   Gary C. Howard (The Gladstone Institutes, San Francisco, California, USA) ,  Matthew R. Kaser (Bell & Associates, San Francisco, California, USA)
Publisher:   Taylor & Francis Inc
Imprint:   CRC Press Inc
Dimensions:   Width: 15.60cm , Height: 2.10cm , Length: 23.50cm
Weight:   0.567kg
ISBN:  

9780849335280

ISBN 10:   0849335280
Pages:   408
Publication Date:   01 October 2006
Audience:   General/trade ,  College/higher education ,  Professional and scholarly ,  General ,  Undergraduate
Replaced By:   9781439869086
Format:   Paperback
Publisher's Status:   Out of Stock Indefinitely
Availability:   In Print  
Limited stock is available. It will be ordered for you and shipped pending supplier's limited stock.

Table of Contents

Antibodies; Matthew R. Kaser and Gary C. Howard Antigens; Paul Algate, Jory Baldridge, and Sally Mossman Adjuvants; Jory Baldridge, Paul Algate, and Sally Mossman Production of Polyclonal Antibodies; Lon V. Kendall Production of Monoclonal Antibodies; Kathleen C. F. Sheehan Quantitative Production of Monoclonal Antibodies; David A. Fox and Elizabeth M. Smith Purification and Characterization of Antibodies; Joseph P. Chandler Making Antibodies in Bacteria; Frederic A. Fellouse and Sachdev S. Sidhu Chemical and Proteolytic Modification of Antibodies; George P. Smith Applications; Lee Bendickson and Marit Nilsen-Hamilton Immunohistochemical Methods; Jose A. Ramos-Vara and Julie Ackerman Saettele Immunoelectron Microscopy; Sara E. Miller and David N. Howell Flow Cytometry; Kristi R. Harkins and M. Elaine Kunze ELISAs; John Chen and Gary C. Howard Antibodies in the Future: Challenges and Opportunities; Matthew R. Kaser and Gary C. Howard

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