Overview

This volume highlights molecular methods to study the phytopathogenic rice blast fungus Magnaporthe oryzae. Chapters in this book cover the history, development, and evolution of the pathogen; molecular methods to increase the knowledge of the biology, genetic, and metabolic diversity of the pathogen; and the pathogen’s adaptability. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls.  Cutting-edge and comprehensive, Magnaporthe oryzae: Methods and Protocols is a valuable resource for any scientist or researcher interested in learning more about this developing field. 

Full Product Details

Author:   Stefan Jacob
Publisher:   Springer-Verlag New York Inc.
Imprint:   Springer-Verlag New York Inc.
Edition:   1st ed. 2021
Volume:   2356
Weight:   0.663kg
ISBN:  

9781071616123

ISBN 10:   1071616129
Pages:   234
Publication Date:   09 July 2021
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

The Impact of Blast Disease: Past, Present, and Future.- The Biology of Invasive Growth by the Rice Blast Fungus Magnaporthe oryzae.- Analysis of the Secondary Metabolism in Magnaporthe oryzae.- The Determination of Carbohydrates by High-Performance Anion-Exchange Chromatography Coupled with Pulsed Amperometric Detection (HPAEC-PAD).- Characterizing the Secretion Systems of Magnaporthe oryzae.- Specimen Preparation and Observations of Magnaporthe oryzae Appressorial Cells Under Electron Microscopy.- 4D Widefield Fluorescence Imaging of Appressorium Morphogenesis by Magnaporthe oryzae.- Tandem Affinity Purification (TAP) of Low Abundance Protein Complexes in Filamentous Fungi Demonstrated using Magnaporthe oryzae.- Quantitative Proteome and Phosphoproteome Profiling in Magnaporthe oryzae.- A Target-Based In Vivo Test System to Identify Novel Fungicides with Mode of Action in the HOG Pathway.- Making Visualization Work for You: Deriving Valuable Insights from Omics Data.- Genome Editing using CRISPR/Cas9 System in the Rice Blast Fungus.- Diurnal, Circadian, and Photomorphogenic Analyses in Magnaporthe oryzae.- Methods to Study Autophagocytosis in Magnaporthe oryzae.- Molecular Detection of Magnaporthe oryzae from Rice Seeds.- Isolation and Functional Analysis of Effector-Proteins of Magnaporthe oryzae.- Analysis of Genetic Variations and Genomic Instabilities in Magnaporthe oryzae.- Controllable Bypass Suppression in Magnaporthe oryzae.

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