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Author:   Karin Öllinger ,  Hanna Appelqvist
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   1st ed. 2017
Volume:   1594
Dimensions:   Width: 17.80cm , Height: 1.90cm , Length: 25.40cm
Weight:   7.611kg
ISBN:  

9781493969326

ISBN 10:   1493969323
Pages:   311
Publication Date:   30 April 2017
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

SILAC-Based Comparative Proteomic Analysis of Lysosomes from Mammalian Cells Using LC-MS/MS.- Quantitative Profiling of Lysosomal Lipidome by Shotgun Lipidomics Analysis of N- And O-Glycosylation of Lysosomal Glycoproteins.- Analyzing Lysosome Related Organelles By Electron Microscopy.- Microscopic Analysis of Lysosomal Membrane Permeabilization.- Quantitative Co-localization and Pattern Analysis Of Endo-Lysosomal Cargo in Subcellular Image Cytometry and Validation on Synthetic Image Sets.- Preparation of a Two-Photon Fluorescent Probe for Imaging H2O2 in Lysosomes in Living Cells and Tissues.- Lysophagy: A Method for Monitoring Lysosomal Rupture Followed by Autophagy Dependent Recovery.- Delivery of Cargo to Lysosomes using Gneosomes.- Lysosomal Acidification in Cultured Astrocytes Using Nanoparticles.- Analysis of Lysosomal pH by Flow Cytometry Using FITC-dextran Loaded Cells.- Detection of Lysosomal Exocytosis in Platelets by Flow Cytometry.- Detection of Lysosomal Exocytosis by Surface Exposure of Lamp1 Luminal Epitopes.- Using the MEROPS Database for Investigation of Lysosomal Peptidases, their Inhibitors and Substrates.- Next Generation Sequencing Approaches to Define the Role of the Autophagy Lysosomal Pathway in Human Disease: The Example of Lysoplex.- Gelatin Zymography using Leupeptin for the Detection of Various Cathepsin L Forms.- Methods for Determination of Α-Glycosidase, Β-Glycosidase, and Α-Galactosidase Activities in Dried Blood Spot Samples.- Prenatal Diagnosis of Lysosomal Storage Disorders Using Chorionic Villi.- Lysosomal Biology in Cancer.  

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