Overview

This detailed book explores methods to isolate, characterize, and investigate key lung innate immune cells. Beginning with an overview, the volume then continues with methods for creating in vitro and in vivo model systems to study inflammatory lung diseases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Lung Innate Immunity and Inflammation: Methods and Protocols aims to be a guidebook and will be of value and interest to researchers investigating innate immunity and inflammation in the lung as well as other organs and tissues.

Full Product Details

Author:   Scott Alper ,  William J. Janssen
Publisher:   Humana Press Inc.
Imprint:   Humana Press Inc.
Edition:   Softcover reprint of the original 1st ed. 2018
Volume:   1809
Weight:   0.846kg
ISBN:  

9781493993291

ISBN 10:   1493993291
Pages:   433
Publication Date:   10 December 2019
Audience:   Professional and scholarly ,  College/higher education ,  Professional & Vocational ,  Postgraduate, Research & Scholarly
Format:   Paperback
Publisher's Status:   Active
Availability:   Manufactured on demand  
We will order this item for you from a manufactured on demand supplier.

Table of Contents

Part I: Lung Innate, Immunity, and Inflammation 1. 500 Million Alveoli from 30,000 Feet: A Brief Primer on Lung Anatomy William McKleroy and Kenneth Lyn-Kew   2. Overview of Innate Lung Immunity and Inflammation David W.H. Riches and Thomas R. Martin   Part II: Isolation and Characterization of Lung Innate Immune Cells 3. Isolation and Characterization of Mononuclear Phagocytes in the Mouse Lung and Lymph Nodes Sophie L. Gibbings and Claudia V. Jakubzick   4. Isolation and Characterization of Mouse Neutrophils Niki D.J. Ubags and Benjamin T. Suratt   5. Isolation and Characterization of Mouse Fibroblasts Benjamin L. Edelman and Elizabeth F. Redente   6. Isolation of Rat and Mouse Alveolar Type II Epithelial Cells Nicole L. Jansing, Jazalle McClendon, Hidenori Kage, Mitsuhiro Sunohara, Juan R. Alvarez, Zea Borok, and Rachel L. Zemans          7. Isolation and Characterization of Human Alveolar type II Cells Beata Kosmider, Robert J. Mason, and Karim Bahmed   8. Air-liquid Interface Culture of Human and Mouse Airway Epithelial Cells Di Jiang, Niccolette Schaefer, and Hong Wei Chu   9. Isolation and Characterization of Human Lung Myeloid Cells Yen-Rei A. Yu and Robert M. Tighe   10. Measurement of Protein Permeability and Fluid Transport of Human Alveolar Epithelial Type II Cells under Pathological Conditions Xiaohui Fang and Michael A. Matthay   11. Measuring Innate Immune Function in Mouse Mononuclear Phagocytes John Matthew Craig and Neil Raj Aggarwal   12. Measuring Neutrophil Bactericidal Activity Kenneth C. Malcolm   13. Modulation of Myeloid Cell Function Using Conditional and Inducible Transgenic Approaches Alexandra L McCubbrey and William J. Janssen   14. Modulation of Lung Epithelial Cell Function Using Conditional and Inducible Transgenic Approaches Adrianne L. Stefanski, Dorota S. Raclawska, and Christopher M. Evans   15. Computational Analysis of RNA-seq Data from Airway Epithelial Cells for Studying Lung Disease Nathan D. Jackson, Lando Ringel, and Max A. Seibold   16. Application of Proteomics in Lung Research Nichole A. Reisdorph, Cole Michel, Kristofer Fritz, and Richard Reisdorph   17. Application of Metabolomics in Lung Research Nichole A. Reisdorph, Charmion Cruickshank-Quinn, Yasmeen Nkrumah-Elie, and Richard Reisdorph   18. Functional Genomics in Murine Macrophages Frank Fang-Yao Lee and Scott Alper   Part III: Analysis of Lung Innate Immunity and Inflammation 19. Assessment of Ozone-induced Lung Injury in Mice Vandy P. Stober and Stavros Garantziotis   20. Lung Histological Methods Aneta Gandjeva and Rubin M. Tuder   21. Intravital Microscopy in the Mouse Lung Yimu Yang, Joseph A Hippensteel, and Eric P Schmidt   22. Mouse Models of Acute Lung Injury and ARDS Franco R. D’Alessio   23. Mouse Models of Asthma Magdalena M. Gorska   24. Animal Models of Pulmonary Fibrosis David N. O’Dwyer and Bethany B. Moore   25. Mouse Models of COPD Karina A. Serban and Irina Petrache   26. Mouse Models of COPD Karina A. Serban and Irina Petrache   27. Delivery of Therapeutics to the Lung Dominique N. Price and Pavan Muttil

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