Low-Cost Methods for Molecular Characterization of Mutant Plants: Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols
Author:   Bradley J. Till ,  Joanna Jankowicz-Cieslak ,  Owen A. Huynh ,  Mayada M. Beshir
Publisher:   Springer International Publishing AG
Edition:   2015 ed.
ISBN:  

9783319162584


Pages:   38
Publication Date:   09 June 2015
Format:   Hardback
Availability:   In Print   Availability explained
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Low-Cost Methods for Molecular Characterization of Mutant Plants: Tissue Desiccation, DNA Extraction and Mutation Discovery: Protocols


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Author:   Bradley J. Till ,  Joanna Jankowicz-Cieslak ,  Owen A. Huynh ,  Mayada M. Beshir
Publisher:   Springer International Publishing AG
Imprint:   Springer International Publishing AG
Edition:   2015 ed.
Dimensions:   Width: 15.50cm , Height: 0.90cm , Length: 23.50cm
Weight:   0.214kg
ISBN:  

9783319162584


ISBN 10:   3319162586
Pages:   38
Publication Date:   09 June 2015
Audience:   Professional and scholarly ,  Professional & Vocational
Format:   Hardback
Publisher's Status:   Active
Availability:   In Print   Availability explained
This item will be ordered in for you from one of our suppliers. Upon receipt, we will promptly dispatch it out to you. For in store availability, please contact us.

Table of Contents

​Introduction.- Health and Safety Considerations.- Sample Collection and Storage.- Low-Cost DNA Extraction.- PCR Amplification for Low-Cost Mutation Discovery.- Enzymatic Mismatch Cleavage and Agarose Gel Evaluation of Samples.- Alternative Enzymology for Mismatch Cleavage for TILLING and Ecotilling: Extraction of Enzymes form Common Weedy Plants.- Example Data.- Conclusions. 

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